{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:09:56Z","timestamp":1792130996329,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T00:00:00Z","timestamp":1360800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"Conditional ligands have enabled the high\u2010throughput production of human leukocyte antigen (HLA<\/jats:styled-content>) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with E<\/jats:styled-content>uropean ancestry, and such tools are scarce for A<\/jats:styled-content>sian HLA<\/jats:styled-content> variants. We report 30 novel irradiation\u2010sensitive ligands, specifically targeting S<\/jats:styled-content>outh E<\/jats:styled-content>ast A<\/jats:styled-content>sian populations, which provide 93, 63, and 79% coverage for HLA<\/jats:styled-content>\u2010A<\/jats:styled-content>, \u2010B<\/jats:styled-content>, and \u2010C<\/jats:styled-content>, respectively. Unique ligands for all 16 HLA<\/jats:styled-content> types were constructed to provide the desired soluble HLA<\/jats:styled-content> product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA<\/jats:styled-content>\u2010based MHC<\/jats:styled-content> stability assay. HLA<\/jats:styled-content> tetramers with redirected specificity could detect antigen\u2010specific CD<\/jats:styled-content>8+<\/jats:sup> T<\/jats:styled-content>\u2010cell responses against human cytomegalovirus, hepatitis B<\/jats:styled-content> (HBV<\/jats:styled-content>), dengue virus (DENV<\/jats:styled-content>), and E<\/jats:styled-content>pstein\u2010B<\/jats:styled-content>arr virus (EBV<\/jats:styled-content>) infections. The potential of this population\u2010centric HLA<\/jats:styled-content> library was demonstrated with the characterization of seven novel T<\/jats:styled-content>\u2010cell epitopes from severe acute respiratory syndrome coronavirus, HBV<\/jats:styled-content>, and DENV<\/jats:styled-content>. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state\u2010of\u2010the\u2010art epitope prediction. This flow cytometry\u2010based technology therefore holds considerable promise for monitoring clinically relevant antigen\u2010specific T<\/jats:styled-content>\u2010cell responses in populations of distinct ethnicity.<\/jats:p>","DOI":"10.1002\/eji.201243088","type":"journal-article","created":{"date-parts":[[2012,12,26]],"date-time":"2012-12-26T14:11:01Z","timestamp":1356531061000},"page":"1109-1120","source":"Crossref","is-referenced-by-count":46,"title":["Conditional ligands for A<\/scp>sian HLA<\/scp> variants facilitate the definition of CD<\/scp>8+<\/sup>T<\/scp>\u2010cell responses in acute and chronic viral diseases"],"prefix":"10.1002","volume":"43","author":[{"given":"Cynthia X. 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