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We used cervical explants to study HIV<\/jats:styled-content>\u20101 transmission, the effects of opsonization on infectivity, and how infection can be prevented. Complement opsonization enhanced HIV<\/jats:styled-content>\u20101 infection of dendritic cells (DC<\/jats:styled-content>s) compared with that by free HIV<\/jats:styled-content>\u20101, but this increased infection was not observed with CD<\/jats:styled-content>4+<\/jats:sup> T<\/jats:styled-content> cells. Blockage of the \u03b14\u2010, \u03b27\u2010, and \u03b21\u2010integrins significantly inhibited HIV<\/jats:styled-content>\u20101 infection of both DC<\/jats:styled-content>s and CD<\/jats:styled-content>4+<\/jats:sup> T<\/jats:styled-content> cells. We found a greater impairment of HIV<\/jats:styled-content>\u20101 infection in DC<\/jats:styled-content>s for complement\u2010opsonized virions compared with that of free virions when \u03b1M<\/jats:styled-content>\/\u03b22\u2010 and \u03b14\u2010integrins were blocked. Blocking the C<\/jats:styled-content>\u2010type lectin receptor macrophage mannose receptor (MMR) inhibited infection of emigrating DC<\/jats:styled-content>s but had no effect on CD<\/jats:styled-content>4+<\/jats:sup> T<\/jats:styled-content>\u2010cell infection. We show that blocking of integrins decreases the HIV<\/jats:styled-content>\u20101 infection of both mucosal DC<\/jats:styled-content>s and CD<\/jats:styled-content>4+<\/jats:sup> T<\/jats:styled-content> cells emigrating from the cervical tissues. These findings may provide the basis of novel microbicidal strategies that may help limit or prevent initial infection of the cervical mucosa, thereby reducing or averting systemic HIV<\/jats:styled-content>\u20101 infection.<\/jats:p>","DOI":"10.1002\/eji.201243257","type":"journal-article","created":{"date-parts":[[2013,5,18]],"date-time":"2013-05-18T21:15:04Z","timestamp":1368911704000},"page":"2361-2372","source":"Crossref","is-referenced-by-count":25,"title":["Blocking of integrins inhibits HIV<\/scp>\u20101 infection of human cervical mucosa immune cells with free and complement\u2010opsonized virions"],"prefix":"10.1002","volume":"43","author":[{"given":"Veronica","family":"Tjomsland","sequence":"first","affiliation":[{"name":"Division of Molecular Virology Department of Clinical and Experimental Medicine Link\u00f6ping University Link\u00f6ping 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