{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:28:31Z","timestamp":1791178111341,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T00:00:00Z","timestamp":1372204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft DFG","award":["Pi295\/7-1"],"award-info":[{"award-number":["Pi295\/7-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The thymus is a primary lymphoid organ responsible for production and selection of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells. Nonetheless, mature <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells and in particular activated <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells can reenter the thymus. Here, we identified memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells specific for lymphocytic choriomeningitis virus or vaccinia virus in the thymus of mice long\u2010time after the infection. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells were mainly located in the thymic medulla, but also in the cortical areas. Interestingly, virus\u2010specific memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells in the thymus expressed the cell surface markers <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>69 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>103 that are characteristic of tissue\u2010resident memory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells in a time\u2010dependent manner. Kinetic analyses and selective depletion of peripheral <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells by antibodies further revealed that thymic virus\u2010specific memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells did not belong to the circulating pool of lymphocytes. Finally, we demonstrate that these thymus\u2010resident virus\u2010specific memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells efficiently mounted a secondary proliferative response, exhibited immediate effector functions and were able to protect the thymus from lymphocytic choriomeningitis virus reinfection. In conclusion, the present study not only describes for the first time virus\u2010specific memory <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells with characteristics of tissue\u2010resident memory T (T<jats:sub>RM<\/jats:sub>) cells in a primary lymphoid organ but also extends our knowledge about local <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell immunity in the thymus.<\/jats:p>","DOI":"10.1002\/eji.201343519","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T09:44:48Z","timestamp":1369820688000},"page":"2295-2304","source":"Crossref","is-referenced-by-count":42,"title":["Thymus\u2010resident memory <scp>CD<\/scp>8<sup>+<\/sup><scp>T<\/scp>\u00a0cells mediate local immunity"],"prefix":"10.1002","volume":"43","author":[{"given":"Maike","family":"Hofmann","sequence":"first","affiliation":[{"name":"Department of Immunology Institute of Medical Microbiology and Hygiene University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Oschowitzer","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Medical Microbiology and Hygiene University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan R.","family":"Kurzhals","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Medical Microbiology and Hygiene University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline C.","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Medical Microbiology and Hygiene University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[{"name":"Department of Immunology Institute of Medical Microbiology and Hygiene University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/44385"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058867"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00103-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.08.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201041377"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010201107"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2079"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1718"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107200108"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102243"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.03.001"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00111-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.5.3155-3160.1992"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/244301a0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.08.006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/342559a0"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.159.2.436"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/300262a0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.5.1039"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.2.379"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601040103"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02786432"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.4049\/jimmunol.146.1.293","article-title":"Entry of antivirally active T lymphocytes into the thymus of virus\u2010infected mice","volume":"146","author":"Gossmann J.","year":"1991","journal-title":"J. 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