{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:55:16Z","timestamp":1791255316316,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,14]],"date-time":"2013-10-14T00:00:00Z","timestamp":1381708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>Mast cells are abundantly situated at contact sites between the body and its environment, such as the skin and, especially during certain immune responses, at mucosal surfaces. They mediate allergic reactions and degrade toxins as well as venoms. However, their roles during innate and adaptive immune responses remain controversial and it is likely that major functions remain to be discovered. Recent developments in mast cell\u2010specific conditional gene targeting in the mouse promise to enhance our understanding of these fascinating cells. To complete the genetic toolbox to study mast cell development, homeostasis and function, it is imperative to inducibly manipulate their gene expression. Here, we report the generation of a novel knock\u2010in mouse line expressing a tamoxifen\u2010inducible version of the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>re recombinase from within the endogenous c\u2010<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>it locus. We demonstrate highly efficient and specific inducible expression of a fluorescent reporter protein in mast cells both in vivo and in vitro. Furthermore, induction of diphtheria toxin A expression allowed selective and efficient ablation of mast cells at various anatomical locations, while other hematopoietic cells remain unaffected. This novel mouse strain will hence be very valuable to study mast cell homeostasis and how specific genes influence their functions in physiology and pathology.<\/jats:p>","DOI":"10.1002\/eji.201343731","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T15:56:45Z","timestamp":1381247805000},"page":"296-306","source":"Crossref","is-referenced-by-count":34,"title":["<scp>C<\/scp>re<scp>ER<\/scp><sup>T2<\/sup> expression from within the c\u2010<scp>K<\/scp>it gene locus allows efficient inducible gene targeting in and ablation of mast cells"],"prefix":"10.1002","volume":"44","author":[{"given":"Klaus","family":"Heger","sequence":"first","affiliation":[{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Seidler","sequence":"additional","affiliation":[{"name":"Klinikum rechts der Isar Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Christoph","family":"Vahl","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Infection Biology Institute of Clinical Microbiology Immunology and Hygiene Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maike","family":"Kober","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Klein","sequence":"additional","affiliation":[{"name":"Klinikum rechts der Isar Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Voehringer","sequence":"additional","affiliation":[{"name":"Department of Infection Biology Institute of Clinical Microbiology Immunology and Hygiene Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Saur","sequence":"additional","affiliation":[{"name":"Klinikum rechts der Isar Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Schmidt\u2010Supprian","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2755"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/381075a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/381077a0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1128\/iai.41.1.445-447.1983","article-title":"Delayed expulsion of adult \n                     Trichinella spiralis by mast cell\u2010deficient W\/Wv mice","volume":"41","author":"Ha T. 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