{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:37:14Z","timestamp":1790534234658,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T00:00:00Z","timestamp":1388102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>Riboflavin, also known as vitamin B<jats:sub>2<\/jats:sub>, is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adults and elderly individuals, who are at the coincidental risk for listeriosis. To address the impact of acute riboflavin deficiency on host defense against <jats:italic>Listeria monocytogenes<\/jats:italic> (L.m.), we generated conditional RFK knockout (KO) strains of mice. Phagocyte\u2010specific RFK KO impaired the capability of phagocytes to control intracellular L.m., which corresponded to a greater susceptibility of mice to in vivo challenge with L.m. The oxidative burst of RFK\u2010deficient phagocytes in response to L.m. infection was significantly reduced. Mechanistically, TNF\u2010induced priming of Nox2, which is needed for oxidative burst, was defective in RFK\u2010deficient phagocytes. Lack of riboflavin in wild\u2010type macrophages for only 6 h shut down TNF\u2010induced, RFK\u2010mediated de novo FMN\/FAD generation, which was accompanied by diminished ROS production and impaired anti\u2010listerial activity. Vice versa, ROS production by riboflavin\u2010deprived macrophages was rapidly restored by riboflavin supplementation. Our results suggest that acute riboflavin deficiency immediately impairs priming of Nox2, which is of crucial relevance for an effective phagocytic immune response in vivo.<\/jats:p>","DOI":"10.1002\/eji.201343940","type":"journal-article","created":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T11:36:06Z","timestamp":1385379366000},"page":"728-741","source":"Crossref","is-referenced-by-count":79,"title":["Riboflavin (vitamin B<sub>2<\/sub>) deficiency impairs NADPH oxidase 2 (Nox2) priming and defense against <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"44","author":[{"given":"Michael","family":"Schramm","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Wiegmann","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Schramm","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Gluschko","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Herb","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Uterm\u00f6hlen","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"},{"name":"Center for Molecular Medicine Cologne (CMMC) University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kr\u00f6nke","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology Immunology and Hygiene University of Cologne  Cologne Germany"},{"name":"Center for Molecular Medicine Cologne (CMMC) University of Cologne  Cologne Germany"},{"name":"Cologne Cluster of Excellence in Cellular Stress Responses in Aging\u2010Associated Diseases (CECAD) University of Cologne  Cologne Germany"},{"name":"German Center for Infection Research Cologne  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-009-0169-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.1999.10718846"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/77.6.1352"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00044.2005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3423"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000136357"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-008-0118-3"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08206"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1820-1823.2004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.4049\/jimmunol.85.5.511","article-title":"The effects of riboflavin deficiency on phagocytosis and susceptibility to infection","volume":"85","author":"Wertman K. 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