{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:48:20Z","timestamp":1791402500737,"version":"4.3.3"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T00:00:00Z","timestamp":1409875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R01TW6612-5"],"award-info":[{"award-number":["5R01TW6612-5"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>During oral infection, mucosal immunity assumes a predominant role. Here, we addressed the role of mast cells (MCs), which are mainly located in mucosa during oral infection with <jats:italic>Toxoplasma gondii<\/jats:italic>, using MC\u2010deficient (W\/W<jats:sup>v<\/jats:sup>) mice. We show that in the absence of MCs the resistance of W\/W<jats:sup>v<\/jats:sup> mice to oral infection was considerably reduced. W\/W<jats:sup>v<\/jats:sup> mice uniformly succumbed within 15 days of infection after administration of cysts of the ME49 strain of <jats:italic>T. gondii<\/jats:italic>. The rapid lethality of <jats:italic>T. gondii<\/jats:italic> in W\/W<jats:sup>v<\/jats:sup> mice correlated with a delayed Th1\u2010cell response, since IFN\u2010\u03b3 and IL\u201012 levels peaked in the later phase of the infection. In vitro, BM\u2010derived MCs were able to recognize parasite lysate in a MyD88\u2010dependent way, reaffirming the role of this TLR adapter in immune responses to <jats:italic>T. gondii<\/jats:italic>. The importance of MCs in vivo was confirmed when W\/W<jats:sup>v<\/jats:sup> mice reconstituted with BM\u2010derived MCs from control mice retrieved an early strong Th1\u2010cell response and specially a significant IL\u201012 production. In conclusion, MCs play an important role for the development of a protective immune response during oral infection with <jats:italic>T. gondii<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/eji.201344185","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:39:22Z","timestamp":1407199162000},"page":"2949-2954","source":"Crossref","is-referenced-by-count":14,"title":["Mast cells are crucial in the resistance against <i>Toxoplasma gondii<\/i> oral infection"],"prefix":"10.1002","volume":"44","author":[{"given":"Aline","family":"Cruz","sequence":"first","affiliation":[{"name":"School of Medicine Federal University of Minas Gerais  Belo Horizonte MG Brazil"},{"name":"Department of Biochemistry and Immunology Institute of Biological Sciences Federal University of Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica Ara\u00fajo","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology Ren\u00e9 Rachou Research Center Oswaldo Cruz Foundation  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vin\u00edcius Melo","family":"de Andrade","sequence":"additional","affiliation":[{"name":"School of Medicine Federal University of Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanuza Chagas do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"School of Medicine Federal University of Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane T.","family":"Cartelle","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Biological Sciences Federal University of Minas Gerais  Belo Horizonte MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa M. 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