{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:03:47Z","timestamp":1775977427958,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T00:00:00Z","timestamp":1410825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"German Science Foundation","award":["FOR832"],"award-info":[{"award-number":["FOR832"]}]},{"name":"Interdisciplinary Clinical Research Center Erlangen"},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["PAS_241506"],"award-info":[{"award-number":["PAS_241506"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Velocigene at Regeneron Inc","award":["U01HG004085"],"award-info":[{"award-number":["U01HG004085"]}]},{"name":"CSD Consortium","award":["U01HG004080"],"award-info":[{"award-number":["U01HG004080"]}]},{"name":"KOMP Repository at UC Davis and CHORI","award":["U42RR024244"],"award-info":[{"award-number":["U42RR024244"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>Activated B\u00a0cells are selected for in germinal centers by regulation of their apoptosis. The Ca<jats:sup>2+<\/jats:sup>\u2010binding cytoskeletal adaptor protein Swiprosin\u20101\/EFhd2 (EFhd2) can promote apoptosis in activated B\u00a0cells. We therefore hypothesized that EFhd2 might limit humoral immunity by repressing both the germinal center reaction and the expected enhancement of immune responses in the absence of EFhd2. Here, we established EFhd2<jats:sup>\u2212\/\u2212<\/jats:sup> mice on a C57BL\/6 background, which revealed normal B\u2010 and T\u2010cell development, basal Ab levels, and T\u2010cell independent type 1, and T\u2010cell independent type 2 responses. However, T\u00a0cell\u2010dependent immunization with sheep red blood cells and infection with the helminth <jats:italic>Nippostrongylus brasiliensis (N.b)<\/jats:italic> increased production of antibodies of multiple isotypes, as well as germinal center formation in EFhd2<jats:sup>\u2212\/\u2212<\/jats:sup> mice. In addition, serum IgE levels and numbers of IgE<jats:sup>+<\/jats:sup> plasma cells were strongly increased in EFhd2<jats:sup>\u2212\/\u2212<\/jats:sup> mice, both after primary as well as after secondary <jats:italic>N.b<\/jats:italic> infection. Finally, mixed bone marrow chimeras unraveled an EFhd2\u2010dependent B\u00a0cell\u2010intrinsic contribution to increased IgE plasma cell numbers in <jats:italic>N.b\u2010<\/jats:italic>infected mice. Hence, we established a role for EFhd2 as a negative regulator of germinal center\u2010dependent humoral type 2 immunity, with implications for the generation of IgE.<\/jats:p>","DOI":"10.1002\/eji.201444479","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:59:22Z","timestamp":1407200362000},"page":"3206-3219","source":"Crossref","is-referenced-by-count":17,"title":["Swiprosin\u20101\/EFhd2 limits germinal center responses and humoral type 2 immunity"],"prefix":"10.1002","volume":"44","author":[{"given":"Sebastian","family":"Brachs","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology Department of Internal Medicine III Nikolaus Fiebiger Center Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Turqueti\u2010Neves","sequence":"additional","affiliation":[{"name":"Division of Infection Biology Institute of Microbiology Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merle","family":"Stein","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology Department of Internal Medicine III Nikolaus Fiebiger Center Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothea","family":"Reimer","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology Department of Internal Medicine III Nikolaus Fiebiger Center Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bent","family":"Brachvogel","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Medical Faculty University of Cologne  Cologne Germany"},{"name":"Center for Biochemistry Medical Faculty University of Cologne  Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"B\u00f6sl","sequence":"additional","affiliation":[{"name":"Institute of Experimental Biomedicine University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Winkler","sequence":"additional","affiliation":[{"name":"Chair of Genetics Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Voehringer","sequence":"additional","affiliation":[{"name":"Division of Infection Biology Institute of Microbiology Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Martin","family":"J\u00e4ck","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology Department of Internal Medicine III Nikolaus Fiebiger Center Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Mielenz","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology Department of Internal Medicine III Nikolaus Fiebiger Center Universit\u00e4tsklinikum Erlangen and Friedrich\u2010Alexander University Erlangen\u2010Nuremberg  Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.000525"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.1009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/342385a0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/353765a0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1794"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061964"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.1.75"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.0112.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2494(05)80006-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v27.i4.60"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00937.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/381751a0"},{"key":"e_1_2_7_14_1","first-page":"429","article-title":"Germinal centers","volume":"30","author":"Victora G. D.","year":"2012","journal-title":"Annu."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.10.032"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115723"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2256"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.02.009"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03278.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.12.006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131539"},{"key":"e_1_2_7_22_1","first-page":"80","article-title":"To B or not to B: B\u00a0cells and the Th2\u2010type immune response to helminths","volume":"32","author":"Harris N.","year":"2010","journal-title":"Trends"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34261"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213368"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.6.3508"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903642"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2012.01.004"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-9-2"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300779"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2013.13.2.55"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22307"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402206"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.3.1858"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(89)90235-9"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394300-2.00004-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-010-0073-2"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1447-5"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102233"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067746"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737314"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.02.001"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838456"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003412"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70511-7"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1547"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2001.00824.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1820"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242780"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-09-379412"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.011"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.101118898"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201444479","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201444479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T20:58:15Z","timestamp":1696539495000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201444479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,16]]},"references-count":51,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1002\/eji.201444479"],"URL":"https:\/\/doi.org\/10.1002\/eji.201444479","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,16]]}}}