{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T16:20:07Z","timestamp":1776270007320,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,10,22]],"date-time":"2014-10-22T00:00:00Z","timestamp":1413936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"JSPS KAKENHI","award":["23590504"],"award-info":[{"award-number":["23590504"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> induces the formation of inflammasomes and subsequent caspase\u20101 activation, and the adaptor apoptosis\u2010associated speck\u2010like protein containing a CARD (ASC) is crucial for this response. However, the role of ASC in <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection in vivo is unclear. In this study, we demonstrate that ASC has a detrimental effect on host defense against <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection at a lethal dose (10<jats:sup>6<\/jats:sup> CFU), but not at a sublethal dose (10<jats:sup>3<\/jats:sup> CFU). During lethal <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection, serum levels of IL\u201018 and IL\u201010 were markedly elevated in WT mice, but not in ASC KO mice. IL\u201018 KO mice were more resistant to lethal <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection than WT mice and had lower levels of serum IL\u201010. Furthermore, blockade of IL\u201010 receptor resulted in a reduction in bacterial counts, suggesting that ASC and IL\u201018 might exacerbate <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection through induction of IL\u201010. We noticed that maturation of IL\u201018 during lethal infection was partially independent of caspase\u20101, but was critically dependent on ASC. ASC was required for the elevation of serum neutrophil serine protease activity, which correlated with caspase\u20101\u2010independent IL\u201018 maturation and IL\u201010 production. Collectively, these results suggest that ASC plays a detrimental role in lethal <jats:italic>L.\u00a0monocytogenes<\/jats:italic> infection through IL\u201018 production in an inflammasome\u2010dependent and \u2010independent manner.<\/jats:p>","DOI":"10.1002\/eji.201444673","type":"journal-article","created":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T05:21:15Z","timestamp":1411449675000},"page":"3696-3707","source":"Crossref","is-referenced-by-count":20,"title":["The adaptor ASC exacerbates lethal <i>Listeria monocytogenes<\/i> infection by mediating IL\u201018 production in an inflammasome\u2010dependent and \u2010independent manner"],"prefix":"10.1002","volume":"44","author":[{"given":"Kohsuke","family":"Tsuchiya","sequence":"first","affiliation":[{"name":"Department of Microbiology Kyoto University Graduate School of Medicine  Kyoto 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