{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:33:30Z","timestamp":1773423210870,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T00:00:00Z","timestamp":1426118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC 306\/1 and 2"],"award-info":[{"award-number":["EXC 306\/1 and 2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC\/SFB832, project A14"],"award-info":[{"award-number":["CRC\/SFB832, project A14"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of L\u00fcbeck, Schwerpunktprogramm Autoimmunit\u00e4t"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>The role of mast cells (MCs) in autoimmunity is the matter of an intensive scientific debate. Based on observations in different MC\u2010deficient mouse strains, MCs are considered as fundamental players in autoimmune diseases. However, most recent data suggest that the outcome of such diseases is strongly affected by the individual mouse strain used. By the use of two <jats:italic>c\u2010Kit<\/jats:italic> mutant MC\u2010deficient mouse strains and one <jats:italic>c\u2010Kit<\/jats:italic>\u2010independent strain, we here investigated the role of MCs in a systemic Ab transfer model of epidermolysis bullosa acquisita, a subepidermal autoimmune blistering skin disease characterized by autoantibodies against type VII collagen. While C57BL\/6J\u2010<jats:italic>Kit<jats:sup>W\u2010sh\/W\u2010sh<\/jats:sup><\/jats:italic> mice developed an unexpected increased blistering phenotype, no significant differences to WT controls were seen in WBB6F<jats:sub>1<\/jats:sub>\u2010<jats:italic>Kit<jats:sup>W\/W\u2010v<\/jats:sup><\/jats:italic> or the novel <jats:italic>Mcpt5\u2010Cre iDTR<\/jats:italic> animals. Interestingly, in a local Ab transfer model, which induces a localized disease, we showed that application of high concentrations of anti\u2010COL7 (where COL7 is type VII collagen) Abs induced MC activation and MC\u2010dependent edema formation that did, however, not contribute to blister induction. Our results indicate that in the autoimmune disorder epidermolysis bullosa acquisita MCs do not contribute to the immune\u2010mediated tissue injury. Modern <jats:italic>c\u2010Kit<\/jats:italic> mutant\u2010independent MC\u2010deficient mouse strains will help to further redefine the role of MCs in autoimmunity.<\/jats:p>","DOI":"10.1002\/eji.201444769","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T10:06:56Z","timestamp":1423822016000},"page":"1462-1470","source":"Crossref","is-referenced-by-count":26,"title":["Conditional depletion of mast cells has no impact on the severity of experimental epidermolysis bullosa acquisita"],"prefix":"10.1002","volume":"45","author":[{"given":"Anika","family":"Kasprick","sequence":"first","affiliation":[{"name":"Priority Area Asthma and Allergy Members of the German Center for Lung Research, Research Center Borstel  Borstel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Yu","sequence":"additional","affiliation":[{"name":"Priority Area Asthma and Allergy Members of the German Center for Lung Research, Research Center Borstel  Borstel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Scholten","sequence":"additional","affiliation":[{"name":"Quintiles GmbH  Neu\u2010Isenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Cologne  Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hendri H.","family":"Pas","sequence":"additional","affiliation":[{"name":"Centre of Blistering Diseases, Department of Dermatology University of Groningen  Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Detlef","family":"Zillikens","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf J.","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Petersen","sequence":"additional","affiliation":[{"name":"Priority Area Asthma and Allergy Members of the German Center for Lung Research, Research Center Borstel  Borstel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198404193101602"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113373"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.06.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00799.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12096"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1065"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200521386"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23702.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2327"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12378589"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.120601.141025"},{"key":"e_1_2_7_13_1","first-page":"965","article-title":"The c\u2010kit receptor, stem cell factor, and mast cells. What each is teaching us about the others","volume":"142","author":"Galli S. J","year":"1993","journal-title":"Am. J. Pathol."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V52.2.447.447"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62055-X"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.09.015"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-007-9153-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.03.028"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.5.813"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11494"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071391"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090320"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2157"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01324"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073176"},{"key":"e_1_2_7_26_1","first-page":"35","article-title":"Mast cells in allergy and autoimmunity: implications for adaptive immunity","volume":"315","author":"Gregory G. D","year":"2006","journal-title":"Methods Mol. Biol."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1443.023"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2011.02.009"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003603"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.3"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb08305.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.3.390"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.3.705"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.3754"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.11.004"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.07.007"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00147"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003642"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080407"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth762"},{"key":"e_1_2_7_41_1","first-page":"191","article-title":"Genetically mast\u2010cell\u2010deficient W\/Wv and Sl\/Sld mice. Their value for the analysis of the roles of mast cells in biologic responses in vivo","volume":"127","author":"Galli S. J.","year":"1987","journal-title":"Am. J. Pathol."},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116856"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197802232980803"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.3.1025"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02136.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10346.x"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201444769","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201444769","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201444769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T18:23:14Z","timestamp":1696616594000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201444769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,12]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/eji.201444769"],"URL":"https:\/\/doi.org\/10.1002\/eji.201444769","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,12]]}}}