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Here, we show that a small peptide derived from the rabies virus glycoprotein fused to protamine residues (RVG\u2010P) can target DNA to myeloid cells, including DCs, which results in enhanced humoral and T\u2010cell responses. DCs targeted with a DNA vaccine encoding the immunodominant vaccinia <jats:italic>B8R<\/jats:italic> gene via RVG\u2010P were able to restimulate vaccinia\u2010specific memory T cells in vitro. Importantly, a single i.v. injection of <jats:italic>B8R<\/jats:italic> gene bound to RVG\u2010P was able to prime a vaccinia\u2010specific T\u2010cell response that was able to rapidly clear a subsequent vaccinia challenge in mice. Moreover, delivery of DNA in DCs was enough to induce DC maturation and efficient Ag presentation without the need for adjuvants. Finally, immunization of mice with a DNA\u2010vaccine encoding West Nile virus (WNV) prM and E proteins via RVG\u2010P elicited high titers of WNV\u2010neutralizing Abs that protected mice from lethal WNV challenge. Thus, RVG\u2010P provides a reagent to target DNA vaccines to myeloid cells and elicit robust T\u2010cell and humoral immune responses.<\/jats:p>","DOI":"10.1002\/eji.201445010","type":"journal-article","created":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T08:06:59Z","timestamp":1412150819000},"page":"82-88","source":"Crossref","is-referenced-by-count":8,"title":["Targeting DNA vaccines to myeloid cells using a small peptide"],"prefix":"10.1002","volume":"45","author":[{"given":"Chunting","family":"Ye","sequence":"first","affiliation":[{"name":"Center of Excellence in Infectious Disease Research Department of Biomedical Sciences Paul L. Foster School of Medicine Texas Tech University Health Sciences Center  El Paso TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jang Gi","family":"Choi","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infectious Disease Research Department of Biomedical Sciences Paul L. Foster School of Medicine Texas Tech University Health Sciences Center  El Paso TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sojan","family":"Abraham","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infectious Disease Research Department of Biomedical Sciences Paul L. Foster School of Medicine Texas Tech University Health Sciences Center  El Paso TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Premlata","family":"Shankar","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infectious Disease Research Department of Biomedical Sciences Paul L. 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