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AMD3100 also mobilizes most mature leukocyte subsets to blood; however, their source and trafficking potential have not been fully delineated. Here, we show that a single injection of AMD3100 10 mg\/kg into C57Bl\/6 mice rapidly mobilizes (peak \u223c 2.5 h) the same leukocyte subsets to blood as in humans. Using this model, we found that AMD3100 mobilization of neutrophils, lymphocytes, and monocytes to blood is not reduced by splenectomy or by blockade of lymphocyte egress from lymph node with FTY720, but is coupled to (i) reduced content of each of these cell types in the bone marrow; (ii) reduced T\u2010cell numbers in thymuses; (iii) increased lymphocytes in lymph nodes; and (iv) increased neutrophil and monocyte content in the lung. Direct intrathymic labeling showed that AMD3100 selectively mobilizes na\u00efve thymic CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells to blood. Finally, AMD3100\u2010induced neutrophil mobilization to blood did not reduce neutrophil trafficking to thioglycollate\u2010inflamed peritoneum. Thus, AMD3100 redistributes lymphocytes, monocytes, and neutrophils from primary immune organs to secondary immune organs, peripheral tissues, and blood, without compromising neutrophil trafficking to inflamed sites.<\/jats:p>","DOI":"10.1002\/eji.201445245","type":"journal-article","created":{"date-parts":[[2015,3,20]],"date-time":"2015-03-20T16:27:50Z","timestamp":1426868870000},"page":"1855-1867","source":"Crossref","is-referenced-by-count":80,"title":["CXCR4 antagonist AMD3100 redistributes leukocytes from primary immune organs to secondary immune organs, lung, and blood in mice"],"prefix":"10.1002","volume":"45","author":[{"given":"Qian","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanzhuo","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Liang","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuzhou","family":"Wan","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundar","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Research Technologies Branch National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda MD 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