{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T01:37:40Z","timestamp":1780623460125,"version":"3.54.1"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,14]],"date-time":"2015-02-14T00:00:00Z","timestamp":1423872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SP583\/2-3"],"award-info":[{"award-number":["SP583\/2-3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"TRC","award":["RC\/MED\/MICR\/14\/1"],"award-info":[{"award-number":["RC\/MED\/MICR\/14\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>The human MHC class I protein HLA\u2010B*27:05 is statistically associated with ankylosing spondylitis, unlike HLA\u2010B*27:09, which differs in a single amino acid in the F pocket of the peptide\u2010binding groove. To understand how this unique amino acid difference leads to a different behavior of the proteins in the cell, we have investigated the conformational stability of both proteins using a combination of in silico and experimental approaches. Here, we show that the binding site of B*27:05 is conformationally disordered in the absence of peptide due to a charge repulsion at the bottom of the F pocket. In agreement with this, B*27:05 requires the chaperone protein tapasin to a greater extent than the conformationally stable B*27:09 in order to remain structured and to bind peptide. Taken together, our data demonstrate a method to predict tapasin dependence and physiological behavior from the sequence and crystal structure of a particular class I allotype.<\/jats:p>\n                  <jats:p>\n                    Also watch the\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.youtube.com\/watch?v=F7UKhYqU79A\">Video Abstract<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1002\/eji.201445307","type":"journal-article","created":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T07:31:16Z","timestamp":1421998276000},"page":"1248-1257","source":"Crossref","is-referenced-by-count":52,"title":["F pocket flexibility influences the tapasin dependence of two differentially disease\u2010associated MHC Class I proteins"],"prefix":"10.1002","volume":"45","author":[{"given":"Esam T.","family":"Abualrous","sequence":"first","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"},{"name":"Department of Physics Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Fritzsche","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Hein","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Al\u2010Balushi","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"},{"name":"Department of Microbiology and Immunology Sultan Qaboos University  Muscat Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Reinink","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"},{"name":"Department of Infectious Diseases and Immunology Faculty of Veterinary Medicine Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise H.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Division of Immunology Department of Pathology Cambridge University  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Wellbrock","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony N.","family":"Antoniou","sequence":"additional","affiliation":[{"name":"University of East London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Springer","sequence":"additional","affiliation":[{"name":"Department of Life Science and Chemistry Jacobs University Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.10.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2013.07.012"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01676-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095910"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2004.100"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2006.10.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22807"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200007000-00001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00865.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10127"},{"key":"e_1_2_7_12_1","first-page":"1111","article-title":"HLA\u2010B27 subtypes positively and negatively associated with spondyloarthropathy","volume":"24","author":"Nasution A. 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