{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:39:17Z","timestamp":1790807957343,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T00:00:00Z","timestamp":1429228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Interdiscipilnary Center for Clinical Research Medical Faculty University of Wuerzburg","award":["A-169"],"award-info":[{"award-number":["A-169"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Though mostly defective, human endogenous retroviruses (HERV) can retain open reading frames, which are especially expressed in the placenta. There, the envelope (env) proteins of HERV\u2010W (Syncytin\u20101), HERV\u2010FRD (Syncytin\u20102), and HERV\u2010K (HML\u20102) were implicated in tolerance against the semi\u2010allogenic fetus. Here, we show that the known HERV env\u2010binding receptors ASCT\u20101 and \u20102 and MFSD2 are expressed by DCs and T\u2010cells. When used as effectors in coculture systems, CHO cells transfected to express Syncytin\u20101, \u20102, or HML\u20102 did not affect T\u2010cell expansion or overall LPS\u2010driven phenotypic DC maturation, however, promoted release of IL\u201012 and TNF\u2010\u03b1 rather than IL\u201010. In contrast, HERV env expressing choriocarcinoma cell lines suppressed T\u2010cell proliferation and LPS\u2010induced TNF\u2010\u03b1 and IL\u201012 release, however, promoted IL\u201010 accumulation, indicating that these effects might not rely on HERV env interactions. However, DCs conditioned by choriocarcinoma, but also transgenic CHO cells failed to promote allogenic T\u2010cell expansion. This was associated with a loss of DC\/T\u2010cell conjugate frequencies, impaired Ca<jats:sup>2+<\/jats:sup> mobilization, and aberrant patterning of f\u2010actin and tyrosine phosphorylated proteins in T\u2010cells. Altogether, these findings suggest that HERV env proteins target T\u2010cell activation indirectly by modulating the stimulatory activity of DCs.<\/jats:p>","DOI":"10.1002\/eji.201445366","type":"journal-article","created":{"date-parts":[[2015,3,9]],"date-time":"2015-03-09T06:25:03Z","timestamp":1425882303000},"page":"1748-1759","source":"Crossref","is-referenced-by-count":50,"title":["Human endogenous retrovirus envelope proteins target dendritic cells to suppress T\u2010cell activation"],"prefix":"10.1002","volume":"45","author":[{"given":"Jonas","family":"Hummel","sequence":"first","affiliation":[{"name":"Institute for Virology and Immunobiology University of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"K\u00e4mmerer","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology University of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute for Virology and Immunobiology University of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elita","family":"Avota","sequence":"additional","affiliation":[{"name":"Institute for Virology and Immunobiology University of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibylle","family":"Schneider\u2010Schaulies","sequence":"additional","affiliation":[{"name":"Institute for Virology and Immunobiology University of Wuerzburg  Wuerzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404838101"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83534-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302972"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199804)20:4<307::AID-BIES7>3.0.CO;2-M"},{"key":"e_1_2_7_6_1","first-page":"1","article-title":"Human endogenous retrovirus K (HERV\u2010K) is expressed in villous and extravillous cytotrophoblast cells of the human placenta","volume":"91","author":"Kammerer U.","year":"2011","journal-title":"J. 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