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Wnt signaling is important to maintain HSC integrity. However, the paracrine role of Wnt proteins in osteoblasts\u2010supported HSC maintenance and differentiation remains unclear. Here, we investigated hematopoiesis in mice with <jats:italic>Wntless<\/jats:italic> (<jats:italic>Wls<\/jats:italic>) deficiency in osteoblasts or Nestin\u2010positive mesenchymal progenitor cells, which presumptively block Wnt secretion in osteoblasts. We detected defective B\u2010cell lymphopoiesis and abnormal T\u2010cell infiltration in the bone marrow of <jats:italic>Wls<\/jats:italic> mutant mice. Notably, no impact on HSC frequency and repopulation in the bone marrow was observed with the loss of osteoblastic <jats:italic>Wls<\/jats:italic>. Our findings revealed a supportive role of Wnts in osteoblasts\u2010regulated B\u2010cell lymphopoiesis. They also suggest a preferential niche role of osteoblastic Wnts for lymphoid cells rather than HSCs, providing new clues for the molecular nature of distinct niches occupied by different hematopoietic cells.<\/jats:p>","DOI":"10.1002\/eji.201445405","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T14:59:27Z","timestamp":1436885967000},"page":"2650-2660","source":"Crossref","is-referenced-by-count":20,"title":["Ablation of <i>Wntless<\/i> in endosteal niches impairs lymphopoiesis rather than HSCs maintenance"],"prefix":"10.1002","volume":"45","author":[{"given":"Jingjing","family":"Cao","sequence":"first","affiliation":[{"name":"Bio\u2010X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Bio\u2010X 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