{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:38:04Z","timestamp":1788871084487,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T00:00:00Z","timestamp":1429228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHM2440-3"],"award-info":[{"award-number":["SCHM2440-3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>\n                    Replication\u2010deficient recombinant adenoviruses are potent vectors for the efficient transient expression of exogenous genes in resting immune cells. However, most leukocytes are refractory to efficient adenoviral transduction as they lack expression of the coxsackie\/adenovirus receptor (CAR). To circumvent this obstacle, we generated the R26\/CAG\u2010CAR\u03941\n                    <jats:sup>StopF<\/jats:sup>\n                    (where R26 is ROSA26 and CAG is CMV early enhancer\/chicken \u03b2 actin promoter) knock\u2010in mouse line. This strain allows monitoring of in situ Cre recombinase activity through expression of CAR\u03941. Simultaneously, CAR\u03941 expression permits selective and highly efficient adenoviral transduction of immune cell populations, such as mast cells or T cells, directly ex vivo in bulk cultures without prior cell purification or activation. Furthermore, we show that CAR\u03941 expression dramatically improves adenoviral infection of in vitro differentiated conventional and plasmacytoid dendritic cells (DCs), basophils, mast cells, as well as Hoxb8\u2010immortalized hematopoietic progenitor cells. This novel dual function mouse strain will hence be a valuable tool to rapidly dissect the function of specific genes in leukocyte physiology.\n                  <\/jats:p>","DOI":"10.1002\/eji.201545457","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T06:54:07Z","timestamp":1426575247000},"page":"1614-1620","source":"Crossref","is-referenced-by-count":15,"title":["A novel Cre recombinase reporter mouse strain facilitates selective and efficient infection of primary immune cells with adenoviral vectors"],"prefix":"10.1002","volume":"45","author":[{"given":"Klaus","family":"Heger","sequence":"first","affiliation":[{"name":"Hematology and Oncology Klinikum rechts der Isar, Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maike","family":"Kober","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Klinikum rechts der Isar, Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rie\u00df","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Klinikum rechts der Isar, Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Drees","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Klinikum rechts der Isar, Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"de Vries","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria  Klosterneuburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Bertossi","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Roers","sequence":"additional","affiliation":[{"name":"Institute for Immunology Technische Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sixt","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria  Klosterneuburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Schmidt\u2010Supprian","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Klinikum rechts der Isar, Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"Max Planck Institute of Biochemistry  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2509"},{"key":"e_1_2_6_3_1","first-page":"e50455","article-title":"Adenoviral transduction of naive CD4 T cells to study Treg differentiation","volume":"78","author":"Warth S. 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