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The fact that the transcription repressors B\u2010cell lymphoma\u20106 and Blimp\u20101 determine lineages of Tfh cells and other types of effector CD4<jats:sup>+<\/jats:sup> T\u00a0cells, respectively, suggests that there are unique mechanisms to establish Tfh\u2010cell identity. In this study, we found that Tfh cells preferentially express the transcriptional coactivator Bob1. Bob1 of Tfh cells was dispensable for the expression of B\u2010cell lymphoma\u20106 and the functional property of the cells for B cell help. However, upon initial immunization of foreign antigens, the percentages of Tfh cells in Bob1<jats:sup>\u2212\/\u2212<\/jats:sup> mice were much higher than those in wild\u2010type (WT) mice. In addition, expansion of Tfh cells within Bob1<jats:sup>\u2212\/\u2212<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T\u00a0cells transferred into WT mice revealed that the high frequency of Tfh cells was caused by a T\u2010cell\u2010intrinsic mechanism. These findings were further supported by the results of in vitro studies demonstrating that Bob1<jats:sup>\u2212\/\u2212<\/jats:sup> Tfh cells had greater proliferative activity in response to stimuli by CD3\/CD28 monoclonal antibody and were also refractory to CD3\u2010induced cell death in comparison to WT Tfh cells. 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