{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:27:11Z","timestamp":1744072031359},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T00:00:00Z","timestamp":1431388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Ectopic gene expression studies in primary immune cells have been notoriously difficult to perform due to the limitations in conventional transfection and viral transduction methods. Although replication\u2010defective adenoviruses provide an attractive alternative for gene delivery, their use has been hampered by the limited susceptibility of murine leukocytes to adenoviral infection, due to insufficient expression of the human coxsackie\/adenovirus receptor (CAR). In this issue of the <jats:italic>European Journal of Immunology<\/jats:italic>, Heger et\u00a0al. [<jats:italic>Eur. J. Immunol<\/jats:italic>. 2015. 45: 1614\u20131620] report the generation of transgenic mice that enable conditional Cre\/loxP\u2010mediated expression of human CAR. The authors demonstrate that this R26\/CAG\u2010CAR\u22061<jats:sup>StopF<\/jats:sup> mouse strain facilitates the faithful monitoring of Cre activity in situ as well as the specific and efficient adenoviral transduction of primary immune cell populations in vitro. Further tweaking of the system towards more efficient gene transfer in vivo remains a future challenge.<\/jats:p>","DOI":"10.1002\/eji.201545685","type":"journal-article","created":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T09:57:51Z","timestamp":1429610271000},"page":"1596-1599","source":"Crossref","is-referenced-by-count":2,"title":["Surmounting limited gene delivery into primary immune cell populations: Efficient cell type\u2010specific adenoviral transduction by CAR"],"prefix":"10.1002","volume":"45","author":[{"given":"Bj\u00f6rn E.","family":"Clausen","sequence":"first","affiliation":[{"name":"Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg\u2010University Mainz  55131 Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Brand","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg\u2010University Mainz  55131 Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalad","family":"Karram","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg\u2010University Mainz  55131 Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,12]]},"reference":[{"key":"e_1_2_4_2_1","first-page":"1399","article-title":"New approaches to the development of adenoviral dendritic cell vaccines in melanoma","volume":"11","author":"Butterfield L. 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