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One of these, the Ski2\u2010like DExH\u2010box helicase DDX60, was recently implicated in resistance of human cells to hepatitis C virus, as well as in induction of IFN\u2010\u03b1\/\u03b2 by retinoic acid inducible gene 1\u2010like receptors (RLRs) that detect the presence of RNA viruses in a cell\u2010intrinsic manner. Here, we sought to investigate the role of DDX60 in IFN\u2010\u03b1\/\u03b2 induction and in resistance to virus infection. Analysis of fibroblasts and myeloid cells from <jats:italic>Ddx60<\/jats:italic>\u2010deficient mice revealed no impairment in IFN\u2010\u03b1\/\u03b2 production in response to RLR agonists, RNA viruses, or other stimuli. Moreover, overexpression of DDX60 did not potentiate IFN induction and DDX60 did not interact with RLRs or capture RLR agonists from virally infected cells. We also failed to identify any impairment in Ddx60\u2010deficient murine cells or mice in resistance to infection with influenza A virus, encephalomyocarditis virus, Sindbis virus, vaccinia virus, or herpes simplex virus\u20101. These results put in question the reported role of DDX60 as a broad\u2010acting positive regulator of RLR responses and hint at the possibility that it may function as a restriction factor highly specific for a particular virus or class of viruses.<\/jats:p>","DOI":"10.1002\/eji.201545794","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T09:39:39Z","timestamp":1441877979000},"page":"3386-3403","source":"Crossref","is-referenced-by-count":34,"title":["Mouse superkiller\u20102\u2010like helicase DDX60 is dispensable for type I IFN induction and immunity to multiple viruses"],"prefix":"10.1002","volume":"45","author":[{"given":"Delphine","family":"Goubau","sequence":"first","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemarthe G.","family":"van der Veen","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Probir","family":"Chakravarty","sequence":"additional","affiliation":[{"name":"Bioinformatics The Francis Crick Institute, Lincoln's Inn Fields Laboratory  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongtuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Molecular Oncology Group Lady Davis Institute\u2014Jewish General Hospital, McGill University  Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Rogers","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Rehwinkel","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safia","family":"Deddouche","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Rosewell","sequence":"additional","affiliation":[{"name":"Transgenic Services The Francis Crick Institute, Clare Hall Laboratory  Potters Bar Herts UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Hiscott","sequence":"additional","affiliation":[{"name":"Vaccine &amp; Gene Therapy Institute of Florida Port Saint Lucie  FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caetano","family":"Reis e Sousa","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory The Francis Crick Institute Lincoln's Inn Fields Laboratory  44 Lincoln's Inn Fields London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.05.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.11.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.12.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1779"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.06.015"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.03.040"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1244040"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12862"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08476"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.04.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04734"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.04.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060792"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022637199"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2013.03.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838832"},{"key":"e_1_2_7_19_1","first-page":"743","volume-title":"Fields virology","author":"Knipe D. 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