{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:58:27Z","timestamp":1790416707737,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T00:00:00Z","timestamp":1465344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sport, and Culture","doi-asserted-by":"crossref","award":["25461715"],"award-info":[{"award-number":["25461715"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>Innate immunity, which is unable to discriminate self from allo\u2010antigens, is thought to be important players in the induction of miscarriages. Here, we show that the administration of IL\u201012 to syngeneic\u2010mated C57BL\/6 mice on gestation day 7.5 (Gd 7.5), drives significant miscarriages in pregnant females. Furthermore, the administration on Gd 7.5 of \u03b1\u2010galactosylceramide (\u03b1\u2010GalCer), which is known to activate invariant natural killer T (iNKT) cells, induced miscarriages in both syngeneic\u2010mated C57BL\/6 mice and allogeneic\u2010mated mice (C57BL\/6 (\u2640) \u00d7 BALB\/c (\u2642)). Surprisingly, the percentages of both DEC\u2010205<jats:sup>+<\/jats:sup> DCs and CD1d\u2010restricted NK1.1<jats:sup>+<\/jats:sup> iNKT cells were higher in the myometrium of pregnant mice treated i.p. with \u03b1\u2010GalCer than in the decidua. IL\u201012 secreted from \u03b1\u2010GalCer\u2010activated DEC\u2010205<jats:sup>+<\/jats:sup> DCs stimulated the secretion of cytokines, including IL\u20102, IL\u20104, IFN\u2010\u03b3, TNF\u2010\u03b1, perforin, and granzyme B, from the NK1.1<jats:sup>+<\/jats:sup> iNKT cells in the myometrium, leading to fetal loss in pregnant mice. Finally, the i.p. administration of IL\u201012 and\/or \u03b1\u2010GalCer in iNKT\u2010deficient J\u03b118(\u2010\/\u2010) (J\u03b118 KO) mice did not induce miscarriages. This study provides a new perspective on the importance of the myometrium, rather than the decidua, in regulating pregnancy and a mechanism of miscarriage mediated by activated DEC\u2010205<jats:sup>+<\/jats:sup> DCs and NK1.1<jats:sup>+<\/jats:sup> iNKT cells in the myometrium of pregnant mice.<\/jats:p>","DOI":"10.1002\/eji.201545923","type":"journal-article","created":{"date-parts":[[2016,5,21]],"date-time":"2016-05-21T15:06:17Z","timestamp":1463843177000},"page":"1867-1877","source":"Crossref","is-referenced-by-count":29,"title":["\u03b1\u2010Galactosylceramide\u2010activated murine NK1.1<sup>+<\/sup> invariant\u2010NKT cells in the myometrium induce miscarriages in mice"],"prefix":"10.1002","volume":"46","author":[{"given":"Tomoko","family":"Ichikawa","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Nippon Medical School  Tokyo Japan"},{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Negishi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Nippon Medical School  Tokyo Japan"},{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masumi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Takeshita","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5236.630"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2012.01.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/383041b0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.7.1121"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20102555"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5248"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.10.5690"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103440"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.6.2091"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5343.1626"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.8.1529"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.740"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2096"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511025103"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5343.1623"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20061551"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.12.004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3369"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0890447"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2004.05.007"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-100003"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110866"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/330484a0"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq224"},{"key":"e_1_2_7_26_1","first-page":"2062","article-title":"Dendritic cell entrapment within the pregnant uterus inhibits immune surveillance of the maternal\/fetal interface in mice","volume":"119","author":"Collins M. 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