{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:15:06Z","timestamp":1792080906460,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T00:00:00Z","timestamp":1453248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Swiss National Foundation","award":["310030.146187\/1"],"award-info":[{"award-number":["310030.146187\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>\n                    Infection of C57BL\/6 mice with most\n                    <jats:italic>Leishmania major<\/jats:italic>\n                    strains results in a healing lesion and clearance of parasites from the skin. Infection of C57BL\/6 mice with the\n                    <jats:italic>L. major<\/jats:italic>\n                    Seidman strain (\n                    <jats:italic>Lm<\/jats:italic>\n                    Sd), isolated from a patient with chronic lesions, despite eliciting a strong Th1 response, results in a nonhealing lesion, poor parasite clearance, and complete destruction of the ear dermis. We show here that in comparison to a healing strain,\n                    <jats:italic>Lm<\/jats:italic>\n                    Sd elicited early upregulation of IL\u20101\u03b2 mRNA and IL\u20101\u03b2\u2010producing dermal cells and prominent neutrophil recruitment to the infected skin. Mice deficient in Nlrp3, apoptosis\u2010associated speck\u2010like protein containing a caspase recruitment domain, or caspase\u20101\/11, or lacking IL\u20101\u03b2 or IL\u20101 receptor signaling, developed healing lesions and cleared\n                    <jats:italic>Lm<\/jats:italic>\n                    Sd from the infection site. Mice resistant to\n                    <jats:italic>Lm<\/jats:italic>\n                    Sd had a stronger antigen\u2010specific Th1 response. The possibility that IL\u20101\u03b2 might act through neutrophil recruitment to locally suppress immunity was supported by the healing observed in neutropenic\n                    <jats:italic>Genista<\/jats:italic>\n                    mice. Secretion of mature IL\u20101\u03b2 by\n                    <jats:italic>Lm<\/jats:italic>\n                    Sd\u2010infected macrophages in vitro was dependent on activation of the Nlrp3 inflammasome and caspase\u20101. These data reveal that Nlrp3 inflammasome\u2010dependent IL\u20101\u03b2, associated with localized neutrophil recruitment, plays a crucial role in the development of a nonhealing form of cutaneous leishmaniasis in conventionally resistant mice.\n                  <\/jats:p>","DOI":"10.1002\/eji.201546015","type":"journal-article","created":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T05:05:55Z","timestamp":1450760755000},"page":"897-911","source":"Crossref","is-referenced-by-count":138,"title":["The Nlrp3 inflammasome, IL\u20101\u03b2, and neutrophil recruitment are required for susceptibility to a nonhealing strain of\n                    <i>Leishmania major<\/i>\n                    in C57BL\/6 mice"],"prefix":"10.1002","volume":"46","author":[{"given":"Melanie","family":"Charmoy","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin P.","family":"Hurrell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry WHO\u2010Immunology Research and Training Center University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Romano","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Ribeiro\u2010Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Riteau","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Mayer\u2010Barber","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Tacchini\u2010Cottier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry WHO\u2010Immunology Research and Training Center University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Sacks","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri933"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/515297"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"3977","DOI":"10.4049\/jimmunol.1003588","article-title":"IL\u201027 and IL\u201021 are associated with T cell IL\u201010 responses in human visceral leishmaniasis","volume":"186","author":"Ansari N. 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