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Phagocytic cells play a crucial part in the prevention of sepsis by clearing bacteria through host innate receptors. Here, we used a phage display library to identify two peptides in <jats:italic>Escherichia coli<\/jats:italic> that interact with host innate receptors. One of these peptides, encoded by the <jats:italic>wzxE<\/jats:italic> gene of <jats:italic>E. coli<\/jats:italic> K\u201012, was involved in the transbilayer movement of a trisaccharide\u2010lipid intermediate in the assembly of enterobacterial common antigen. Peptide\u2010receptor interactions induced CD16\u2010mediated inhibitory immunoreceptor tyrosine\u2010based activating motif signaling, blocking the production of ROS and bacterial killing. This CD16\u2010mediated inhibitory signaling was abrogated in a WzxE<jats:sup>\u2212\/\u2212<\/jats:sup> mutant of <jats:italic>E. coli<\/jats:italic> K\u201012, restoring the production of ROS and bacterial killing. Taken together, the two novel CD16 ligands identified negatively regulate bacterial killing and inflammation. Our findings may contribute toward the development of new immunotherapies for <jats:italic>E. coli<\/jats:italic>\u2010mediated infectious diseases and inflammation.<\/jats:p>","DOI":"10.1002\/eji.201546118","type":"journal-article","created":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T10:20:30Z","timestamp":1464258030000},"page":"1926-1935","source":"Crossref","is-referenced-by-count":8,"title":["Negative regulation of bacterial killing and inflammation by two novel CD16 ligands"],"prefix":"10.1002","volume":"46","author":[{"given":"Jaqueline","family":"Beppler","sequence":"first","affiliation":[{"name":"Emergency Medicine Department University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanae Ben","family":"Mkaddem","sequence":"additional","affiliation":[{"name":"Inserm Unit 1149 and ERL CNRS 8252 Center for Research on Inflammation University Paris Diderot  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussara","family":"Michaloski","sequence":"additional","affiliation":[{"name":"Vascular Biology Laboratory Chemistry Institute University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Vargas","family":"Honorato","sequence":"additional","affiliation":[{"name":"Biosciences National Laboratory (LNBio)  Campinas, Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irineu Tadeu","family":"Velasco","sequence":"additional","affiliation":[{"name":"Emergency Medicine Department University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S\u00e9rgio Lopes","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Biosciences National Laboratory (LNBio)  Campinas, Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Jos\u00e9","family":"Giordano","sequence":"additional","affiliation":[{"name":"Vascular Biology Laboratory Chemistry Institute University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato C.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Inserm Unit 1149 and ERL CNRS 8252 Center for Research on Inflammation University Paris Diderot  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano","family":"Pinheiro da Silva","sequence":"additional","affiliation":[{"name":"Emergency Medicine Department University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.010"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07201"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.12.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00020"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920312804871148"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161208786264124"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(05)89002-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00079"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a002352"},{"key":"e_1_2_7_11_1","first-page":"131","article-title":"Fc receptors as adaptive immunoreceptors","volume":"382","author":"Daeron M.","year":"2014","journal-title":"Curr. 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