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This bacterium infects and proliferates mainly in macrophages and dendritic cells, where it is recognized by pattern recognition receptors (PRRs) including Nod\u2010like receptors (NLRs). Our group recently demonstrated the role of AIM2 and NLRP3 in <jats:italic>Brucella<\/jats:italic> recognition. Here, we investigated the participation of NLRP12 in innate immune response to <jats:italic>B. abortus<\/jats:italic>. We show that NLRP12 inhibits the early production of IL\u201012 by bone marrow\u2010derived macrophages upon <jats:italic>B. abortus<\/jats:italic> infection. We also observed that NLRP12 suppresses in vitro NF\u2010\u03baB and MAPK signaling in response to <jats:italic>Brucella<\/jats:italic>. Moreover, we show that NLRP12 modulates caspase\u20101 activation and IL\u20101\u03b2 secretion in <jats:italic>B. abortus<\/jats:italic> infected\u2010macrophages. Furthermore, we show that mice lacking NLRP12 are more resistant in the early stages of <jats:italic>B. abortus<\/jats:italic> infection: NLRP12<jats:sup>\u2212\/\u2212<\/jats:sup> infected\u2010mice have reduced bacterial burdens in the spleens and increased production of IFN\u2010\u03b3 and IL\u20101\u03b2 compared with wild\u2010type controls. In addition, NLRP12 deficiency leads to reduction in granuloma number and size in mouse livers. Altogether, our findings suggest that NLRP12 plays an important role in negatively regulating the early inflammatory responses against <jats:italic>B. abortus<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/eji.201646502","type":"journal-article","created":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T05:52:57Z","timestamp":1477979577000},"page":"51-59","source":"Crossref","is-referenced-by-count":46,"title":["NLRP12 negatively regulates proinflammatory cytokine production and host defense against <i>Brucella abortus<\/i>"],"prefix":"10.1002","volume":"47","author":[{"given":"Tatiana N.","family":"Silveira","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco T\u00falio R.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de 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