{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T16:32:47Z","timestamp":1770136367615,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010564","name":"German Center for Lung Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010564","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>Nasopharyngeal colonization with <jats:italic>Streptococcus pneumoniae<\/jats:italic> (Spn) is an important precondition for the development of pneumococcal pneumonia. At the same time, nasopharyngeal colonization with Spn has been shown to mount adaptive immune responses against Spn in mice and humans. Cellular responses of the nasopharyngeal compartment, including the nasal\u2010associated lymphoid tissue, to pneumococcal colonization and their importance for developing adaptive immune responses are poorly defined. We show that nasopharyngeal colonization with <jats:italic>S.\u00a0pneumoniae<\/jats:italic> led to substantial expansion of dendritic cells (DCs) both in nasopharyngeal tissue and nasal\u2010associated lymphoid tissue of mice. Depletion of DCs achieved by either diphtheria toxin (DT) treatment of chimeric zDC<jats:sup>+\/DTR<\/jats:sup> mice, or by use of FMS\u2010like tyrosine kinase 3 ligand (Flt3L) KO mice exhibiting congenitally reduced DC pool sizes, significantly diminished antibody responses after colonization with Spn, along with impaired protective immunity against invasive pneumococcal disease. Collectively, the data show that classical DCs contribute to pneumococcal colonization induced adaptive immune responses against invasive pneumococcal disease in two different mouse models. These data may be useful for future nasopharyngeal vaccination strategies against pneumococcal diseases in humans.<\/jats:p>","DOI":"10.1002\/eji.201646700","type":"journal-article","created":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T17:08:17Z","timestamp":1484845697000},"page":"540-551","source":"Crossref","is-referenced-by-count":13,"title":["Nasopharyngeal colonization with <i>Streptococcus pneumoniae<\/i> triggers dendritic cell dependent antibody responses against invasive disease in mice"],"prefix":"10.1002","volume":"47","author":[{"given":"Anne","family":"Dommaschk","sequence":"first","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meritxell","family":"Tort Tarres","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara F.","family":"Bittersohl","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Maus","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Stolper","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny","family":"Jonigk","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover School of Medicine  Hannover Germany"},{"name":"German Center for Lung Research partner site BREATH  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Braubach","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torsten","family":"Lippmann","sequence":"additional","affiliation":[{"name":"Institute of Pathology Hannover School of Medicine  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Welte","sequence":"additional","affiliation":[{"name":"Clinic for Pneumology Hannover School of Medicine  Hannover Germany"},{"name":"German Center for Lung Research partner site BREATH  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich A.","family":"Maus","sequence":"additional","affiliation":[{"name":"Department of Experimental Pneumology Hannover School of Medicine  Hannover Germany"},{"name":"German Center for Lung Research partner site BREATH  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2009.12.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1871"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12752"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805004012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/381547"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.129502"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00313-13"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7718-7726.2005"},{"key":"e_1_2_7_11_1","first-page":"1899","article-title":"Cellular effectors mediating Th17\u2010dependent clearance of pneumococcal colonization in mice","volume":"119","author":"Zhang Z.","year":"2009","journal-title":"J. Clin. Invest."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.53"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-009-0579-4"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201212-2277OC"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501254102"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5807-5813.2004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.7043-7046.2005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011576"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01972-06"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2010.02.009"},{"key":"e_1_2_7_21_1","first-page":"121","article-title":"Protection of pneumococcal infection by maternal intranasal immunization with pneumococcal surface protein A","volume":"72","author":"Hotomi M.","year":"2011","journal-title":"Adv. Otorhinolaryngol."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201011-1783OC"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00477-10"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00299.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2007.9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002837"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01360-10"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.89"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.135"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20112675"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00854-12"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.11.3489"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2007.6"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.08.089"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1997.d01-159.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.d01-690.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.5.3099"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02500-14"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00084-13"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903794"},{"key":"e_1_2_7_41_1","first-page":"3960","article-title":"Examining the role of nasopharyngeal\u2010associated lymphoreticular tissue (NALT) in mouse responses to vaccines","volume":"66","author":"Cisney E. D.","year":"2012","journal-title":"J. Vis Exp"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.95"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025558"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05286-11"},{"key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.chom.2011.01.007","article-title":"T(H)17\u2010based vaccine design for prevention of Streptococcus pneumoniae colonization","volume":"9","author":"Moffitt K. L.","journal-title":"Cell Host Microbe"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201401-0128OC"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0132OC"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5828"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200610-1533OC"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1086\/376571"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00902-15"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu056"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201646700","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201646700","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:28:59Z","timestamp":1696631339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201646700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/eji.201646700"],"URL":"https:\/\/doi.org\/10.1002\/eji.201646700","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2]]}}}