{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:06:01Z","timestamp":1791324361059,"version":"4.3.1"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T00:00:00Z","timestamp":1504656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["VICI 91814650"],"award-info":[{"award-number":["VICI 91814650"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cross\u2010linking of the IgA Fc receptor (Fc\u03b1RI) by IgA induces release of the chemoattractant LTB4, thereby recruiting neutrophils in a positive feedback loop. IgA autoantibodies of patients with autoimmune blistering skin diseases therefore induce massive recruitment of neutrophils, resulting in severe tissue damage. To interfere with neutrophil mobilization and reduce disease morbidity, we developed a panel of specific peptides mimicking either IgA or Fc\u03b1RI sequences. CLIPS technology was used to stabilize three\u2010dimensional structures and to increase peptides\u2019 half\u2010life. IgA and Fc\u03b1RI peptides reduced phagocytosis of IgA\u2010coated beads, as well as IgA\u2010induced ROS production and neutrophil migration in in vitro and ex vivo (human skin) experiments. Since topical application would be the preferential route of administration, Cetomacrogol cream containing an IgA CLIPS peptide was developed. In the presence of a skin permeation enhancer, peptides in this cream were shown to penetrate the skin, while not diffusing systemically. Finally, epitope mapping was used to discover sequences important for binding between IgA and Fc\u03b1RI. In conclusion, a cream containing IgA or Fc\u03b1RI peptide mimetics, which block IgA\u2010induced neutrophil activation and migration in the skin may have therapeutic potential for patients with IgA\u2010mediated blistering skin diseases.<\/jats:p>","DOI":"10.1002\/eji.201646782","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T07:52:42Z","timestamp":1500882762000},"page":"1835-1845","source":"Crossref","is-referenced-by-count":20,"title":["Peptide mimetics of immunoglobulin A (IgA) and Fc\u03b1RI block IgA\u2010induced human neutrophil activation and migration"],"prefix":"10.1002","volume":"47","author":[{"given":"Marieke H.","family":"Heineke","sequence":"first","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia P.E.","family":"van der Steen","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rianne M.","family":"Korthouwer","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Joris","family":"Hage","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Netherlands Cancer Institute\u2010Antoni van Leeuwenhoek Hospital  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes P.M.","family":"Langedijk","sequence":"additional","affiliation":[{"name":"Pepscan Therapeutics, Zuidersluisweg 2  Lelystad The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris J.","family":"Benschop","sequence":"additional","affiliation":[{"name":"Pepscan Therapeutics, Zuidersluisweg 2  Lelystad The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jantine E.","family":"Bakema","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\/Head\u2010Neck Surgery VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry W.","family":"Slootstra","sequence":"additional","affiliation":[{"name":"Pepscan Therapeutics, Zuidersluisweg 2  Lelystad The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6308-9266","authenticated-orcid":false,"given":"Marjolein","family":"van Egmond","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology VU University Medical Center  Amsterdam The Netherlands"},{"name":"Department of Surgery VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2014.01.047"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/15665240113136660079"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12337"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.06.047"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-020712-164023"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101763"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610-0387.2011.07809_suppl.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-013-1409-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3800"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4666"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.202788"},{"key":"e_1_2_7_13_1","first-page":"800","article-title":"Peptide\u2010mediated desmoglein 3 crosslinking prevents pemphigus vulgaris autoantibody\u2010induced skin blistering","volume":"123","author":"Spindler V.","year":"2013","journal-title":"J. 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