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It is still a matter of debate whether and how NKG2D mediates priming of CD8<jats:sup>+<\/jats:sup> T cells in vivo, due to a lack of studies where NKG2D is eliminated exclusively in these cells. Here, we studied the impact of NKG2D on effector CD8<jats:sup>+<\/jats:sup> T\u2010cell formation. NKG2D deficiency that is restricted to murine CD8<jats:sup>+<\/jats:sup> T cells did not impair antigen\u2010specific T\u2010cell expansion following mouse CMV and lymphocytic choriomeningitis virus infection, but reduced their capacity to produce cytokines. Upon infection, conventional dendritic cells induce NKG2D ligands, which drive cytokine production on CD8<jats:sup>+<\/jats:sup> T cells via the Dap10 signaling pathway. T\u2010cell development, homing, and proliferation were not affected by NKG2D deficiency and cytotoxicity was only impaired when strong T\u2010cell receptor (TCR) stimuli were used. Transfer of antigen\u2010specific CD8<jats:sup>+<\/jats:sup> T cells demonstrated that NKG2D deficiency attenuated their capacity to reduce viral loads. The inability of NKG2D\u2010deficient cells to produce cytokines could be overcome with injection of IL\u201015 superagonist during priming. In summary, our data show that NKG2D has a nonredundant role in priming of CD8<jats:sup>+<\/jats:sup> T cells to produce antiviral cytokines.<\/jats:p>","DOI":"10.1002\/eji.201646805","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T17:45:20Z","timestamp":1491414320000},"page":"1123-1135","source":"Crossref","is-referenced-by-count":16,"title":["NKG2D stimulation of CD8<sup>+<\/sup> T cells during priming promotes their capacity to produce cytokines in response to viral infection in mice"],"prefix":"10.1002","volume":"47","author":[{"given":"Inga","family":"Kavazovi\u0107","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine University of Rijeka  Rijeka Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Lenarti\u0107","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine University of Rijeka  Rijeka 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