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There are two major catalytic subunit genes, <jats:italic>PRKACA<\/jats:italic> and <jats:italic>PRKACB<\/jats:italic> encoding the catalytic subunits C\u03b1 and C\u03b2. The <jats:italic>PRKACB<\/jats:italic> gene encodes several splice variants including C\u03b22, which is enriched in T\u2010, B\u2010 and natural killer cells. C\u03b22 is significantly larger (46\u00a0kDa) than any other C splice variant. In this study we characterized mice ablated for the C\u03b22 protein demonstrating a significantly reduced cAMP\u2010induced catalytic activity of PKA in the spleenocytes, lymphocytes and thymocytes. We also observed a significantly increased number of CD62L\u2010expressing CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells in LNs, accompanied by increased susceptibility to systemic inflammation by the C\u03b22 ablated mice. The latter was reflected in an elevated sensitivity to collagen\u2010induced arthritis (CIA), as well as higher concentration of TNF\u2010\u03b1 and lower concentration of IL\u201010 in response to LPS challenges. We suggest a role of C\u03b22 in regulating innate as well as adaptive immune sensitivity in\u00a0vivo.<\/jats:p>","DOI":"10.1002\/eji.201646809","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T15:22:42Z","timestamp":1503588162000},"page":"1880-1889","source":"Crossref","is-referenced-by-count":23,"title":["Ablation of the C\u03b22 subunit of PKA in immune cells leads to increased susceptibility to systemic inflammation in mice"],"prefix":"10.1002","volume":"47","author":[{"given":"Line Victoria","family":"Moen","sequence":"first","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Sener","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Volchenkov","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"},{"name":"Rheumatech AS  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja Camilla","family":"Svarstad","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aud Marit","family":"Eriksen","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halvor L.","family":"Holen","sequence":"additional","affiliation":[{"name":"Rheumatech AS  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3481-8084","authenticated-orcid":false,"given":"Bj\u00f8rn S.","family":"Sk\u00e5lhegg","sequence":"additional","affiliation":[{"name":"Department of Nutrition Institute of Basic Medical Sciences University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2013.03.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2741\/Skalhegg"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-3-465"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0014-2956.2001.02429.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.16.3.630"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405580101"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6433"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-7-20"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2003.08.014"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.47.29560"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111412200"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.4.1571"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2008.10.013"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glp133"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-3-307"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"15707","DOI":"10.1016\/S0021-9258(19)49593-8","article-title":"Cyclic AMP\u2010dependent protein kinase type I mediates the inhibitory effects of 3',5'\u2010cyclic adenosine monophosphate on cell replication in human T lymphocytes","volume":"267","author":"Skalhegg B. 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