{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:31:05Z","timestamp":1775079065983,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["HBF\u2010131552"],"award-info":[{"award-number":["HBF\u2010131552"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Natural killer (NK) cells are cytotoxic lymphocytes that selectively respond against abnormal cells. Human cytomegalovirus (HCMV) infection causes expansion of NKG2C<jats:sup>+<\/jats:sup>CD57<jats:sup>+<\/jats:sup> NK cells in vivo and NKG2C<jats:sup>+<\/jats:sup> NK cells proliferate when cultured with HCMV\u2010infected cells. This raises the possibility of an NK\u2010cell subset selectively responding against a specific pathogen and accruing memory. To test this possibility, we compared proliferation, natural cytotoxicity and interferon\u2010\u03b3 (IFN\u2010\u03b3) production of NK cells from HCMV\u2010seropositive and HCMV\u2010seronegative individuals co\u2010cultured with HCMV\u2010infected or uninfected MRC\u20105 cells. There was no significant difference in proliferation of NK cells from HCMV\u2010seropositive or seronegative individuals against uninfected MRC\u20105 cells, but significantly more NK cells from the HCMV\u2010seropositive group proliferated in response to HCMV\u2010infected MRC\u20105 cells. Natural cytotoxicity of NK cells against K562 cells increased following co\u2010culture with HCMV\u2010infected versus uninfected MRC\u20105 only for the HCMV\u2010seropositive group. After co\u2010culture with HCMV\u2010infected MRC\u20105 cells, proliferating NK cells from HCMV\u2010seropositive donors selectively produced IFN\u2010\u03b3 when re\u2010exposed to HCMV\u2010infected MRC\u20105 cells. Both NKG2C<jats:sup>+<\/jats:sup> and NKG2C<jats:sup>\u2212<\/jats:sup> NK cells proliferated in co\u2010culture with HCMV\u2010infected MRC\u20105 cells, with the fraction of proliferating NKG2C<jats:sup>+<\/jats:sup> NK cells directly correlating with the circulating NKG2C<jats:sup>+<\/jats:sup> fraction. These data illustrate an at least partly NKG2C\u2010independent human NK\u2010cell memory\u2010type response against HCMV.<\/jats:p>","DOI":"10.1002\/eji.201646819","type":"journal-article","created":{"date-parts":[[2017,5,5]],"date-time":"2017-05-05T15:22:46Z","timestamp":1493997766000},"page":"1032-1039","source":"Crossref","is-referenced-by-count":17,"title":["NK cells generate memory\u2010type responses to human cytomegalovirus\u2010infected fibroblasts"],"prefix":"10.1002","volume":"47","author":[{"given":"Nicholas","family":"Newhook","sequence":"first","affiliation":[{"name":"Immunology and Infectious Diseases Program Division of BioMedical Sciences Faculty of Medicine Memorial University of Newfoundland  St. John's NL Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neva","family":"Fudge","sequence":"additional","affiliation":[{"name":"Immunology and Infectious Diseases Program Division of BioMedical Sciences Faculty of Medicine Memorial University of Newfoundland  St. John's NL Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-2036","authenticated-orcid":false,"given":"Michael","family":"Grant","sequence":"additional","affiliation":[{"name":"Immunology and Infectious Diseases Program Division of BioMedical Sciences Faculty of Medicine Memorial University of Newfoundland  St. John's NL Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1332"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.02.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813192106"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-04-419283"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3227"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2784"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871528113666140211100616"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1060042"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.1.29"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092258599"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070884"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-27743-9_10"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07665"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2233572100"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-282301"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110900108"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-05-2058"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2008.02431.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-386995"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201964"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3682"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01096-13"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI77440"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270517"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/35869"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199809)28:09<2854::AID-IMMU2854>3.0.CO;2-W"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(00)00190-7"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.3.1369"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.01.098"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2015.09.003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-157354"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-372003"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7470124"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(01)00212-1"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.02.008"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1203034"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxs080"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302053"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502438"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-576124"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.04.005"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-84-20714"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201646819","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201646819","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T20:08:59Z","timestamp":1695672539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201646819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,23]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1002\/eji.201646819"],"URL":"https:\/\/doi.org\/10.1002\/eji.201646819","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,23]]}}}