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We evaluated the therapeutic effect of murine u\u2010ADSCs and sorted subsets of u\u2010ADSCs in a concanavalin A (ConA) induced murine model of hepatitis, as well as their characteristics. We found that 10\u201320% of u\u2010ADSCs expressed the CD45 leukocyte\u2010related antigen. CD68, which is a marker of macrophages (M\u03a6s), was expressed by 50% of CD45<jats:sup>+<\/jats:sup> u\u2010ADSCs. About 90% of CD68<jats:sup>+<\/jats:sup>CD45<jats:sup>+<\/jats:sup> cells expressed CD206 antigen, which is a marker of inhibitory M2\u2010type M\u03a6s. Genes related to M2\u2010type MUs were especially more highly expressed by CD45<jats:sup>+<\/jats:sup>CD206<jats:sup>+<\/jats:sup> u\u2010ADSCs than by CD45<jats:sup>\u2212<\/jats:sup> u\u2010ADSCs. CD45<jats:sup>+<\/jats:sup> u\u2010ADSCs inhibited the expression of cytokines\/chemokines and suppressed the proliferation of splenocytes stimulated with ConA. We observed that not only whole u\u2010ADSCs, but also the CD45<jats:sup>+<\/jats:sup> subset of u\u2010ADSCs ameliorated the ConA\u2010induced hepatitis in mice. In conclusion, we show that freshly isolated murine u\u2010ADSCs were effective against acute hepatitis, and CD45<jats:sup>+<\/jats:sup> u\u2010ADSCs acting phenotypically and functionally like M2\u2010type M\u03a6s, contributed to the repair of liver tissue undergoing inflammation.<\/jats:p>","DOI":"10.1002\/eji.201646835","type":"journal-article","created":{"date-parts":[[2017,9,11]],"date-time":"2017-09-11T08:22:43Z","timestamp":1505118163000},"page":"2163-2174","source":"Crossref","is-referenced-by-count":13,"title":["The CD45<sup>+<\/sup> fraction in murine adipose tissue derived stromal cells harbors immune\u2010inhibitory inflammatory cells"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2550-2317","authenticated-orcid":false,"given":"Alessandro","family":"Nasti","sequence":"first","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Sakai","sequence":"additional","affiliation":[{"name":"School of Medicine College of Medical Pharmaceutical and Health Sciences Kanazawa University  Japan"},{"name":"Department of Gastroenterology Kanazawa University Hospital  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Seki","sequence":"additional","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldine Belen","family":"Buffa","sequence":"additional","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Komura","sequence":"additional","affiliation":[{"name":"School of Medicine College of Medical Pharmaceutical and Health Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatsune","family":"Mochida","sequence":"additional","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Yamato","sequence":"additional","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Disease Control and Homeostasis Graduate School of Medical Sciences Kanazawa University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuyen T. 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