{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:13:17Z","timestamp":1790291597720,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T00:00:00Z","timestamp":1517184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006315","name":"Reumafonds","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006315","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004522","name":"Top Institute Pharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004522","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Emerging evidence indicates that a dynamic interplay between the immune system and adipocytes contributes to the disturbed homeostasis in adipose tissue of obese subjects. Recently, we observed IL\u20106\u2010secretion by CD4<jats:sup>+<\/jats:sup> T\u00a0cells from the stromal vascular fraction (SVF) of the infrapatellar fat pad (IFP) of knee osteoarthritis patients directly ex vivo. Here we show that human IL\u20106<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T\u00a0cells from SVF display a more activated phenotype than the IL\u20106<jats:sup>\u2212<\/jats:sup> T\u00a0cells, as evidenced by the expression of the activation marker CD69. Analysis of cytokines secretion, as well as expression of chemokine receptors and transcription factors associated with different Th subsets (Treg, Th1, Th2, Th17 and Tfh) revealed that IL\u20106\u2010secreting CD4<jats:sup>+<\/jats:sup> T\u00a0cells cannot be assigned to a conventional Th subset. TCR\u03b2 gene analysis revealed that IL\u20106<jats:sup>+<\/jats:sup> and IL\u20106<jats:sup>\u2212<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T\u00a0cells appear clonally unrelated to each other, suggesting a different specificity of these cells. In line with these observations, adipocytes are capable of enhancing IL\u20106 production by CD4<jats:sup>+<\/jats:sup> T\u00a0cells. Thus, IL\u20106<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T\u00a0cells are TCR\u03b1\u03b2 T\u00a0cells expressing an activated phenotype potentially resulting from an interplay with adipocytes that could be involved in the inflammatory processes in the OA joint.<\/jats:p>","DOI":"10.1002\/eji.201747037","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T10:52:44Z","timestamp":1514458364000},"page":"471-481","source":"Crossref","is-referenced-by-count":12,"title":["Functional and phenotypical analysis of IL\u20106\u2010secreting CD4<sup>+<\/sup> T\u00a0cells in human adipose tissue"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0732-0491","authenticated-orcid":false,"given":"Anja J.","family":"de Jong","sequence":"first","affiliation":[{"name":"Department of Rheumatology Leiden University Medical Centre, Leiden  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Pollastro","sequence":"additional","affiliation":[{"name":"Department of Experimental immunology Academic Medical Center  Amsterdam The Netherlands"},{"name":"Department of Clinical Immunology &amp; Rheumatology ARC | Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanneke C.","family":"Kwekkeboom","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Leiden University Medical Centre, Leiden  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan N.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Leiden University Medical Centre, Leiden  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemarie L.","family":"Dorj\u00e9e","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Leiden University Medical Centre, Leiden  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleida M.","family":"Bakker","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Leiden University Medical Centre, Leiden  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fawaz","family":"Alzaid","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMRS 1138 Sorbonne Universit\u00e9s  Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Universit\u00e9 Paris Descartes Universit\u00e9 Paris Diderot  Paris France"},{"name":"Centre de Recherche des Cordeliers  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Soprani","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMRS 1138 Sorbonne Universit\u00e9s  Paris France"},{"name":"Clinique Geoffroy Saint\u2010Hilaire Ramsey General de Sant\u00e9  Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Universit\u00e9 Paris Descartes Universit\u00e9 Paris Diderot  Paris France"},{"name":"Centre de Recherche des Cordeliers  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob G.H.H.","family":"Nelissen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan B.","family":"Mullers","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Alrijne Hospital  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Venteclef","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMRS 1138 Sorbonne Universit\u00e9s  Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Universit\u00e9 Paris Descartes Universit\u00e9 Paris Diderot  Paris France"},{"name":"Centre de Recherche des Cordeliers  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niek","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Experimental immunology Academic Medical Center  Amsterdam The Netherlands"},{"name":"Department of Clinical Immunology &amp; 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