{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:27:59Z","timestamp":1761514079774,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T00:00:00Z","timestamp":1511308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MUS\u201094027"],"award-info":[{"award-number":["MUS\u201094027"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Viruses, particularly the Epstein\u2010Barr virus (EBV) has long been suspected to exacerbate acute arthritic symptoms. However, the cell populations that contribute to import viruses into the inflamed tissues remain to be identified. In the present study, we have investigated the role of monocytes in the transport of Murid herpesvirus 68 (MHV\u201068), a mouse virus closely related to EBV, using the serum transfer\u2010induced arthritis (STIA) model. We found compelling evidence that MHV\u201068 infection markedly increased disease severity in NR4A1<jats:sup>\u2212\/\u2212<\/jats:sup> mice, which are deficient for Ly6C<jats:sup>low<\/jats:sup> monocytes. In contrast, the MHV\u201068\u2010induced enhancement of joint inflammation was lessened in CCR2<jats:sup>\u2212\/\u2212<\/jats:sup> mice, suggesting the involvement of inflammatory Ly6C<jats:sup>high<\/jats:sup> monocytes in viral transport. We also observed that following selective depletion of monocyte subsets by administration of clodronate, MHV\u201068 transport into the synovium occurs only in the presence of Ly6C<jats:sup>high<\/jats:sup> monocytes. Tracking of adoptively transferred Ly6C<jats:sup>high<\/jats:sup> GFP infected monocytes into arthritic CCR2<jats:sup>\u2212\/\u2212<\/jats:sup> mice by two\u2010photon intravital microscopy showed that this monocyte subset has the capacity to deliver viruses to inflamed AR joints, as confirmed by the detection of viral DNA in inflamed tissues of recipient mice. We thus conclude that Ly6C<jats:sup>high<\/jats:sup> monocytes import MHV\u201068 when they are mobilized to the inflamed arthritic joint.<\/jats:p>","DOI":"10.1002\/eji.201747048","type":"journal-article","created":{"date-parts":[[2017,10,5]],"date-time":"2017-10-05T07:22:38Z","timestamp":1507188158000},"page":"250-257","source":"Crossref","is-referenced-by-count":6,"title":["Ly6C<sup>high<\/sup> monocytes facilitate transport of Murid herpesvirus 68 into inflamed joints of arthritic mice"],"prefix":"10.1002","volume":"48","author":[{"given":"Manon","family":"LeBel","sequence":"first","affiliation":[{"name":"Laboratory of Innate Immunology Centre de recherche du CHU de Qu\u00e9bec \u2010 Universit\u00e9 Laval  Qu\u00e9bec QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit","family":"Egarnes","sequence":"additional","affiliation":[{"name":"Laboratory of Innate Immunology Centre de recherche du CHU de Qu\u00e9bec \u2010 Universit\u00e9 Laval  Qu\u00e9bec 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