{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:40:19Z","timestamp":1744263619991,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T00:00:00Z","timestamp":1502668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Integrins regulate leukocyte trafficking during homeostasis and inflammatory conditions. However, the role of \u03b14 and \u03b27 integrins in guiding eosinophil transmigration into the lungs during filarial manifestation of Tropical Pulmonary Eosinophilia (TPE) has not been explored. In this study, mice exhibiting TPE manifestations were administered with in vivo neutralizing antibodies against integrins \u03b14 and \u03b27 or their combination and immuno\u2010pathological parameters were evaluated. Results show an intact lung barrier, significantly lower lung inflammation and reduced eosinophil counts in the Bronchoalveolar lavage fluid and lungs of mice receiving anti\u2010\u03b14<jats:sup>+<\/jats:sup>\u03b27 treatment. Reduced eosinophil peroxidase and \u03b2\u2010hexosaminidase activity, downregulation of inflammatory genes, lower production of inflammatory lipid intermediates like prostaglandins E2 and D2, leukotriene B4 and cysteinyl leukotrienes were also noted in anti\u2010\u03b14<jats:sup>+<\/jats:sup>\u03b27 treated mice. Reduced accumulation of central memory, effector memory, regulatory T cells and lower production of IL\u20104, IL\u20105, and TGF\u2010\u03b2 were other cardinal features of anti\u2010\u03b14<jats:sup>+<\/jats:sup>\u03b27 treated mice lungs. Flow cytometry\u2010sorted lung eosinophils from anti\u2010\u03b14<jats:sup>+<\/jats:sup>\u03b27 treated mice showed higher apoptotic potential, downregulated anti\u2010apoptotic gene Bcl\u20102, and exhibited reduced F\u2010actin polymerization and calcium influx as compared to IgG controls. In summary, neutralization of \u03b14<jats:sup>+<\/jats:sup>\u03b27 integrins impairs the transmigration, activation and survival of eosinophils and reduces TPE induced pathology in mice lungs.<\/jats:p>","DOI":"10.1002\/eji.201747086","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T07:22:38Z","timestamp":1500880958000},"page":"1501-1512","source":"Crossref","is-referenced-by-count":9,"title":["In vivo neutralization of \u03b14 and \u03b27 integrins inhibits eosinophil trafficking and prevents lung injury during tropical pulmonary eosinophilia in mice"],"prefix":"10.1002","volume":"47","author":[{"given":"Pankaj","family":"Sharma","sequence":"first","affiliation":[{"name":"Parasitology Division CSIR\u2010Central Drug Research Institute  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aditi","family":"Sharma","sequence":"additional","affiliation":[{"name":"Parasitology Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9324-7843","authenticated-orcid":false,"given":"Mrigank","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Parasitology Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4A0715-309RR"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2003.10.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.d01-713.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb03260.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.6.4741"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-13-94"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0904190"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00140.2015"},{"key":"e_1_2_8_10_1","first-page":"e00818","article-title":"Functional impairment of murine dendritic cell subsets following infection with infective larval stage 3 of Brugia malayi","volume":"85","author":"Sharma A.","year":"2016","journal-title":"Infect. Immun."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0607344"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.3.1672"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283136"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.04850.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02241.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.4440"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.09.006"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02498.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1833"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.03.054"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00179-3"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.07.084"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.alit.2014.11.002","article-title":"Leukotriene E4 induces MUC5AC release from human airway epithelial NCI\u2010H292 cells","volume":"64","author":"Shirasaki H.","journal-title":"Allergol. Int."},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2014.02.006"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003988"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00351.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1002\/eji.200940231","article-title":"Protein kinase C regulates mitochondrial targeting of Nur77 and its family member Nor\u20101 in thymocytes undergoing apoptosis","volume":"40","author":"Thompson J.","journal-title":"Eur. J. Immunol."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2015.03.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05040-11"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1463-1468.2001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02456.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1999.37.2.93"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2014.02.001"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.164.3.2009055"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01060.2005"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.1.23.413k13_23_32"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3963"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00824.x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.11.6128"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102253"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.3.1884"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.04.013"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201747086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201747086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T02:58:00Z","timestamp":1696647480000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201747086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,14]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/eji.201747086"],"URL":"https:\/\/doi.org\/10.1002\/eji.201747086","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"type":"print","value":"0014-2980"},{"type":"electronic","value":"1521-4141"}],"subject":[],"published":{"date-parts":[[2017,8,14]]}}}