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Here we compared the outcome of immunization with the T\u2010cell dependent antigen, NP\u2010conjugated to chicken gamma globulin (NP\u2010CGG) adjuvanted with the toll\u2010like receptor 9 (TLR9) ligands, CpG\u2010A or CpG\u2010B, alone or conjugated with the cationic lipid carrier, DOTAP. We provide evidence that only NP\u2010CGG adjuvanted with DOTAP\u2010CpG\u2010B was an effective vaccine in mice resulting in robust germinal center responses, isotype switching and high affinity NP\u2010specific antibodies. The effectiveness of DOTAP\u2010CpG\u2010B as an adjuvant was dependent on the expression of the TLR9 signaling adaptor MyD88 in immunized mice. These results indicate DOTAP\u2010CpG\u2010B but not DOTAP\u2010CpG\u2010A is an effective adjuvant for T cell\u2010dependent protein antigen\u2010based vaccines.<\/jats:p>","DOI":"10.1002\/eji.201747113","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T10:07:00Z","timestamp":1501582020000},"page":"1890-1899","source":"Crossref","is-referenced-by-count":22,"title":["T cell\u2010dependent antigen adjuvanted with DOTAP\u2010CpG\u2010B but not DOTAP\u2010CpG\u2010A induces robust germinal center responses and high affinity antibodies in mice"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9949-9424","authenticated-orcid":false,"given":"Munir","family":"Akkaya","sequence":"first","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billur","family":"Akkaya","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology National Institute of Allergy and Infectious Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick W.","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Miozzo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirna","family":"Pena","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Feng","family":"Qi","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Manzella\u2010Lapeira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Bolland","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan K.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics National Institute of Allergy and Infectious Diseases National Institutes of Health  Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,21]]},"reference":[{"issue":"2","key":"e_1_2_7_2_1","first-page":"S21","article-title":"Development of a single\u2010shot subunit vaccine for HIV\u20101","volume":"10","author":"Cleland J. 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