{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:43:02Z","timestamp":1790826182374,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1106439"],"award-info":[{"award-number":["1106439"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pressure ulcers are a chronic problem for patients or the elderly who require extended periods of bed rest. The formation of ulcers is due to repeated cycles of ischemia\u2010reperfusion (IR), which initiates an inflammatory response. Advanced ulcers disrupt the skin barrier, resulting in further complications. To date, the immunological aspect of skin IR has been understudied, partly due to the complexity of the skin immune cells. Through a combination of mass cytometry, confocal imaging and intravital multiphoton imaging, this study establishes a workflow for multidimensionality single cell analysis of skin myeloid cell responses in the context of IR injury with high spatiotemporal resolution. The data generated has provided us with previously uncharacterized insights into the distinct cellular behavior of resident dendritic cells (DCs) and recruited neutrophils post IR. Of interest, we observed a drop in DDC numbers in the IR region, which was subsequently replenished 48h post IR. More importantly, in these cells, we observe an attenuated response to repeated injuries, which may have implications in the subsequent wound healing process.<\/jats:p>","DOI":"10.1002\/eji.201747347","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T23:22:36Z","timestamp":1520378556000},"page":"1014-1019","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The impact of ischemia\u2010reperfusion injuries on skin resident murine dendritic cells"],"prefix":"10.1002","volume":"48","author":[{"given":"Chi Ching","family":"Goh","sequence":"first","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"},{"name":"Department of Chemical and Biomolecular Engineering (ChBE) National University of Singapore (NUS)  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilien","family":"Evrard","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu Zhen","family":"Chong","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingrou","family":"Tan","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"},{"name":"Department of Dermatology National Skin Centre  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard De Li","family":"Tan","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Wei Weng","family":"Teng","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Weninger","sequence":"additional","affiliation":[{"name":"Centenary Institute for Cancer Medicine and Cell Biology  Newtown New South Wales Australia"},{"name":"Discipline of Dermatology University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Laurence","family":"Becker","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Liang","family":"Tey","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Skin Centre  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan William","family":"Newell","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering (ChBE) National University of Singapore (NUS)  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1905-3586","authenticated-orcid":false,"given":"Lai Guan","family":"Ng","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) A*STAR (Agency for Science Technology and Research  Biopolis Singapore"},{"name":"Discipline of Dermatology University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASW.0000269314.23015.e9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13671-013-0063-9"},{"key":"e_1_2_8_4_1","first-page":"X","article-title":"Inducing Ischemia\u2010reperfusion Injury in the mouse ear skin for intravital multiphoton imaging of immune responses","volume":"118","author":"Goh C. 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