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As such, the genetically\u2010engineered MCPT8<jats:sup>DTR<\/jats:sup> mouse line has been used to specifically deplete basophils following treatment with diphtheria toxin (DT). In this study, we showed that DT\u2010injected MCPT8<jats:sup>DTR<\/jats:sup> mice exhibited a striking decrease of eosinophils and neutrophils in skin when subjected to a hapten fluorescein isothiocyanate (FITC)\u2010induced allergic contact dermatitis (ACD) experimental protocol. Unexpectedly, we found that loss of skin eosinophils and neutrophils was not due to a lack of basophil\u2010mediated recruitment, as DT injection caused a systemic reduction of eosinophils and neutrophils in MCPT8<jats:sup>DTR<\/jats:sup> mice in a time\u2010dependent manner. Furthermore, we found that hematopoietic stem\u2010cell\u2010derived granulocyte\u2010macrophage progenitors (GMPs) expressed MCPT8 gene, and that these cells were depleted upon DT injection. Finally, we optimized a protocol in which a low\u2010dose DT achieved a better specificity for depleting basophils, but not GMPs, in MCPT8<jats:sup>DTR<\/jats:sup> mice, and demonstrate that basophils do not play a major role in recruiting eosinophils and neutrophils to ACD skin. These data provide new and valuable information about functional studies of basophils.<\/jats:p>","DOI":"10.1002\/eji.201747351","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T09:36:50Z","timestamp":1515577010000},"page":"861-873","source":"Crossref","is-referenced-by-count":17,"title":["Treatment of MCPT8<sup>DTR<\/sup> mice with high\u2010 or low\u2010dose diphtheria toxin leads to differential depletion of basophils and granulocyte\u2010macrophage progenitors"],"prefix":"10.1002","volume":"48","author":[{"given":"Carole","family":"El Hachem","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire CNRS UMR 7104 INSERM U964 Universit\u00e9 de Strasbourg  Illkirch France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Hener","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire 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