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Infection of newborn mice with mouse cytomegalovirus (MCMV) intraperitoneally is a well\u2010established model of congenital human cytomegalovirus infection, which best recapitulates the hematogenous route of virus spread to brain and subsequent pathology. Here, we used this model to investigate the role, dynamics, and phenotype of CD8<jats:sup>+<\/jats:sup> T\u00a0cells in the brain following infection of newborn mice. We show that CD8<jats:sup>+<\/jats:sup> T\u00a0cells infiltrate the brain and form a pool of tissue\u2010resident memory T\u00a0cells (T<jats:sub>RM<\/jats:sub> cells) that persist for lifetime. Adoptively transferred virus\u2010specific CD8<jats:sup>+<\/jats:sup> T\u00a0cells provide protection against primary MCMV infection in newborn mice, reduce brain pathology, and remain in the brain as T<jats:sub>RM<\/jats:sub> cells. Brain CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells were long\u2010lived, slowly proliferating cells able to respond to local challenge infection. Importantly, brain CD8<jats:sup>+<\/jats:sup> T<jats:sub>RM<\/jats:sub> cells controlled latent MCMV and their depletion resulted in virus reactivation and enhanced inflammation in brain.<\/jats:p>","DOI":"10.1002\/eji.201847526","type":"journal-article","created":{"date-parts":[[2018,3,3]],"date-time":"2018-03-03T08:52:41Z","timestamp":1520067161000},"page":"950-964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Brain\u2010resident memory CD8<sup>+<\/sup> T\u00a0cells induced by congenital CMV infection prevent brain pathology and virus reactivation"],"prefix":"10.1002","volume":"48","author":[{"given":"Ilija","family":"Brizi\u0107","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine University of Rijeka  Rijeka Croatia"},{"name":"Center for Proteomics Faculty of Medicine University of Rijeka  Rijeka 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