{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:57:38Z","timestamp":1790834258433,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    During acute viral infections in mice, IL\u20107R\u03b1 and KLRG1 together are used to distinguish the short\u2010lived effector cells (SLEC; IL\u20107R\u03b1\n                    <jats:sup>lo<\/jats:sup>\n                    KLRG\n                    <jats:sup>hi<\/jats:sup>\n                    ) from the precursors of persisting memory cells (MPEC; IL\u20107R\u03b1\n                    <jats:sup>hi<\/jats:sup>\n                    KLRG1\n                    <jats:sup>lo<\/jats:sup>\n                    ). We here show that these markers can be used to define distinct subsets in the circulation and lymph nodes during the acute phase and in \u201csteady state\u201d in humans. In contrast to the T cells in the circulation, T cells derived from lymph nodes hardly contain any KLRG1\u2010expressing cells. The four populations defined by IL\u20107R\u03b1 and KLRG1 differ markedly in transcription factor, granzyme and chemokine receptor expression. When studying renal transplant recipients experiencing a primary hCMV and EBV infection, we also found that after viral control, during latency, Ki\u201067\u2010negative SLEC can be found in the peripheral blood in considerable numbers. Thus, combined analyses of IL\u20107R\u03b1 and KLRG1 expression on human herpes virus\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells can be used to separate functionally distinct subsets in humans. As a noncycling IL\u20107R\u03b1\n                    <jats:sup>lo<\/jats:sup>\n                    KLRG1\n                    <jats:sup>hi<\/jats:sup>\n                    population is abundant in healthy humans, we conclude that this combination of markers not only defines short\u2010lived effector cells during the acute response but also stable effector cells that are formed and remain present during latent herpes infections.\n                  <\/jats:p>","DOI":"10.1002\/eji.201847897","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T01:18:03Z","timestamp":1552958283000},"page":"694-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Expression of IL\u20107R\u03b1 and KLRG1 defines functionally distinct CD8\n                    <sup>+<\/sup>\n                    T\u2010cell populations in humans"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4694-6048","authenticated-orcid":false,"given":"Ester B. M.","family":"Remmerswaal","sequence":"first","affiliation":[{"name":"Department of Experimental Immunology Amsterdam Infection and Immunity Institute Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Renal Transplant Unit Division of Internal Medicine Academic Medical Centre Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pleun","family":"Hombrink","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner laboratory  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Nota","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner laboratory  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1777-9589","authenticated-orcid":false,"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[{"name":"Institute for Immunology University Medical Centre Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ineke J. M.","family":"ten Berge","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology Amsterdam Infection and Immunity Institute Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Renal Transplant Unit Division of Internal Medicine Academic Medical Centre Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 A. W.","family":"van Lier","sequence":"additional","affiliation":[{"name":"Sanquin Research and Landsteiner laboratory  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel C.","family":"van Aalderen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology Amsterdam Infection and Immunity Institute Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Renal Transplant Unit Division of Internal Medicine Academic Medical Centre Amsterdam UMC University of Amsterdam  Amsterdam The 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