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To improve immunization against the persistent health challenge of\n                    <jats:italic>Mtb<\/jats:italic>\n                    infection, we have studied the CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cell response to Bacillus Calmette\u2010Gu\u00e9rin (BCG) and recombinant BCG (rBCG) in mice. Here, we generated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cells with an rBCG\u2010based vaccine encoding the Ag85B protein of\n                    <jats:italic>M. kansasii<\/jats:italic>\n                    , termed rBCG\u2010Mkan85B, followed by boosting with plasmid DNA expressing the Ag85B gene (DNA\u2010Mkan85B). We identified two MHC\u2010I (H2\u2010K\n                    <jats:sup>d<\/jats:sup>\n                    )\u2010restricted epitopes that induce cross\u2010reactive responses to\n                    <jats:italic>Mtb<\/jats:italic>\n                    and other related mycobacteria in both BALB\/c (H2\n                    <jats:sup>d<\/jats:sup>\n                    ) and CB6F1 (H2\n                    <jats:sup>b\/d<\/jats:sup>\n                    ) mice. The H2\u2010K\n                    <jats:sup>d<\/jats:sup>\n                    \u2010restricted peptide epitopes elicited polyfunctional CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cell responses that were also highly cross\u2010reactive with those of other proteins of the Ag85 complex. Tetramer staining indicated that the two H2\u2010K\n                    <jats:sup>d<\/jats:sup>\n                    \u2010restricted epitopes elicit distinct CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cell populations, a result explained by the X\u2010ray structure of the two peptide\/H2\u2010K\n                    <jats:sup>d<\/jats:sup>\n                    complexes. These results suggest that rBCG\u2010Mkan85B vector\u2010based immunization and DNA\u2010Mkan85B boost may enhance CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cell response to\n                    <jats:italic>Mtb<\/jats:italic>\n                    , and might help to overcome the limited effectiveness of the current BCG in eliciting tuberculosis immunity.\n                  <\/jats:p>","DOI":"10.1002\/eji.201847988","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T09:16:56Z","timestamp":1559035016000},"page":"1399-1414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["MHC\u2010restricted Ag85B\u2010specific CD8\n                    <sup>+<\/sup>\n                    T\u00a0cells are enhanced by recombinant BCG prime and DNA boost immunization in mice"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9417-0315","authenticated-orcid":false,"given":"Shihoko","family":"Komine\u2010Aizawa","sequence":"first","affiliation":[{"name":"Division of Microbiology, Department of Pathology and Microbiology Nihon University School of Medicine Nihon University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immune System Biology NIAID National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory  Tokyo Japan"},{"name":"The Research Institute of Tuberculosis Japan Anti\u2010Tuberculosis Association  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology and Microbiology Nihon University School of Medicine Nihon University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory  Tokyo Japan"},{"name":"The Research Institute of Tuberculosis Japan Anti\u2010Tuberculosis Association  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa F.","family":"Boyd","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of Immune System Biology NIAID National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Margulies","sequence":"additional","affiliation":[{"name":"Molecular Biology Section, Laboratory of 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