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This receptor participates in innate immune responses to different pathogens, including the bacteria of genus\n                    <jats:italic>Brucella<\/jats:italic>\n                    . Our group recently demonstrated that\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    \u2010induced IL\u20101\u03b2 secretion involves NLRP3 inflammasome and it is partially dependent on mitochondrial ROS production. However, other factors could be involved, such as P2X7\u2010dependent potassium efflux, membrane destabilization, and cathepsin release. Moreover, there is increasing evidence that nitric oxide acts as a modulator of NLRP3 inflammasome. The aim of this study was to unravel the mechanism of NLRP3 inflammasome activation induced by\n                    <jats:italic>B. abortus<\/jats:italic>\n                    , as well as the involvement of bacterial nitric oxide (NO) as a modulator of this inflammasome pathway. We demonstrated that NO produced by\n                    <jats:italic>B. abortus<\/jats:italic>\n                    can be used by the bacteria to modulate IL\u20101\u03b2 secretion in infected murine macrophages. Additionally, our results suggest that\n                    <jats:italic>B. abortus<\/jats:italic>\n                    \u2010induced IL\u20101\u03b2 secretion depends on a P2X7\u2010independent potassium efflux, lysosomal acidification, cathepsin release, mechanisms clearly associated to NLRP3 inflammasome. In summary, our results help to elucidate the molecular mechanisms of NLRP3 activation and regulation during an intracellular bacterial infection.\n                  <\/jats:p>","DOI":"10.1002\/eji.201848016","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T04:01:35Z","timestamp":1553745695000},"page":"1023-1037","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Brucella abortus<\/i>\n                    nitric oxide metabolite regulates inflammasome activation and IL\u20101\u03b2 secretion in murine macrophages"],"prefix":"10.1002","volume":"49","author":[{"given":"Priscila Carneiro","family":"Campos","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco T\u00falio Ribeiro","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6885-3438","authenticated-orcid":false,"given":"F\u00e1bio Ant\u00f4nio Vitarelli","family":"Marinho","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Gen\u00e9tica Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika Sousa","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariza Gabriela Faleiro","family":"de Moura Lodi Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4062-5577","authenticated-orcid":false,"given":"Sergio Costa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT\u2010DT) Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico Minist\u00e9rio de Ci\u00eancia Tecnologia e Inova\u00e7\u00e3o  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12296"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.03.012"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2231"},{"key":"e_1_2_8_5_1","first-page":"15","article-title":"Targeting the NLRP3 inflammasome in chronic inflammatory diseases: current perspectives","volume":"8","author":"Ozaki E.","year":"2015","journal-title":"J. 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