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Therefore, B lymphocytes and antibody\u2010secreting cells are considered relevant targets for therapies. However, isolation and characterisation of auto\u2010reactive specific B lymphocytes are limited, primarily due to technical issues. In this work, we purified extractable nuclear antigen\u2010specific and citrullinated peptide\u2010specific auto\u2010reactive B lymphocytes by magnetic selection with ENA\u2010 and citrullinated peptide\u2010bound immunobeads. We obtained blood auto\u2010reactive B lymphocytes from most patients. Their nature was primarily na\u00efve B cells, some of them in an active status, with low levels of somatic hypermutations in the immunoglobulin heavy\u2010chain variable regions. Their presence correlated with serum levels of autoAb. Auto\u2010reactive B lymphocytes were able to differentiate into auto\u2010reactive antibody\u2010secreting cells under conditions of stimulation. In addition, based on the presence of circulating auto\u2010reactive B cells and\/or antibody\u2010secreting cells, four different profiles were described in lupus patients. Thus, tracking auto\u2010reactive B cells and\/or antibody\u2010secreting cells in patient blood could represent a biomarker for deciding whether to use therapies blocking either B cells, plasma cells or both, as well as a new tool for monitoring minimal residual autoimmune disease in patients.<\/jats:p>","DOI":"10.1002\/eji.201848065","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T12:46:33Z","timestamp":1553085993000},"page":"1107-1116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Autoreactive B\u2010lymphocytes in SLE and RA patients: Isolation and characterisation using extractable nuclear and citrullinated antigens bound to immunobeads"],"prefix":"10.1002","volume":"49","author":[{"given":"Raquel","family":"de la Varga\u2010Mart\u00ednez","sequence":"first","affiliation":[{"name":"Servicio de Inmunolog\u00eda UGC de Hematolog\u00eda Inmunolog\u00eda y Gen\u00e9tica 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