{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:17:13Z","timestamp":1775978233917,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T00:00:00Z","timestamp":1560816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007255","name":"Bonizzi-Theler Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007255","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008494","name":"Vontobel-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008494","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Protective immunity against intracellular pathogens, including bacteria, usually relies on cellular immunity. However, antibodies are also implicated in mediating protection against intracellular bacteria. In case of airway infection with\n                    <jats:italic>Legionella pneumophila (Lpn)<\/jats:italic>\n                    , the causative agent of Legionnaires' disease, pre\u2010existing\n                    <jats:italic>Lpn<\/jats:italic>\n                    \u2010specific antibodies were shown to afford protection within two days of infection. Here we dissected the early kinetics of Ab\u2010mediated protection against airway\n                    <jats:italic>Lpn<\/jats:italic>\n                    infection and observed two kinetically and mechanistically distinct phases of protection by passively administered antibodies. Within the first hour of infection,\n                    <jats:italic>Lpn<\/jats:italic>\n                    \u2010opsonizing antibodies provided almost 10\u2010fold protection in an antibody Fc\u2010dependent, but FcR\u2010independent manner. Later on, by two days post infection,\n                    <jats:italic>Lpn<\/jats:italic>\n                    \u2010specific Ab\u2010mediated protection strictly involved Fc\u03b3R, Syk kinase activity in alveolar macrophages and induction of reactive oxygen species (ROS). The findings presented here contribute to the understanding of the mechanisms of Ab\u2010mediated control of\n                    <jats:italic>Lpn<\/jats:italic>\n                    infection in actively or passively immunized individuals.\n                  <\/jats:p>","DOI":"10.1002\/eji.201948106","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T08:44:15Z","timestamp":1557477855000},"page":"1415-1420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Two sequential layers of antibody\u2010mediated control of\n                    <i>Legionella pneumophila<\/i>\n                    infection"],"prefix":"10.1002","volume":"49","author":[{"given":"Stefan S.","family":"Weber","sequence":"first","affiliation":[{"name":"Institute of Microbiology ETH Z\u00fcrich  Vladimir\u2010Prelog\u2010Weg 1\u20135\/10 Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Stoycheva","sequence":"additional","affiliation":[{"name":"Institute of Microbiology ETH Z\u00fcrich  Vladimir\u2010Prelog\u2010Weg 1\u20135\/10 Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5418-316X","authenticated-orcid":false,"given":"Falk","family":"Nimmerjahn","sequence":"additional","affiliation":[{"name":"Institute of Genetics University of Erlangen\u2010Nuernberg  Erwin\u2010Rommelstr. 3 Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-2354","authenticated-orcid":false,"given":"Annette","family":"Oxenius","sequence":"additional","affiliation":[{"name":"Institute of Microbiology ETH Z\u00fcrich  Vladimir\u2010Prelog\u2010Weg 1\u20135\/10 Z\u00fcrich 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