{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T18:03:56Z","timestamp":1765994636466,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T00:00:00Z","timestamp":1573603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007793","name":"Stiftelsen Kristian Gerhard Jebsen","doi-asserted-by":"publisher","award":["project SKGJ\u2010MED\u2010017"],"award-info":[{"award-number":["project SKGJ\u2010MED\u2010017"]}],"id":[{"id":"10.13039\/100007793","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Celiac disease (CeD) is driven by CD4<jats:sup>+<\/jats:sup>\u00a0T\u2010cell responses to dietary gluten proteins of wheat, barley, and rye when deamidated gluten epitopes are presented by certain disease\u2010associated HLA\u2010DQ allotypes. About 90% of the CeD patients express HLA\u2010DQ2.5. In such patients, five gluten epitopes dominate the anti\u2010gluten T\u2010cell response; two epitopes unique to wheat, two epitopes present in wheat, barley, and rye and one epitope unique to barley. Despite presence of barley in commonly consumed food and beverages and hence being a prominent source of gluten, knowledge about T\u2010cell responses elicited by barley in CeD is scarce. Therefore, in this study, we explored T\u2010cell response toward the barley unique epitope DQ2.5\u2010hor\u20103 (PIPEQPQPY) by undertaking HLA\u2010DQ:gluten peptide tetramer staining, single\u2010cell T\u2010cell receptor (TCR) \u03b1\u03b2 sequencing, T\u2010cell cloning, and T\u2010cell proliferation studies. We demonstrate that majority of the CeD patients generate T\u2010cell response to DQ2.5\u2010hor\u20103, and this response is characterized by clonal expansion, preferential TCR V\u2010gene usage and public TCR features thus echoing findings previously made for wheat gluten epitopes. The knowledge that biased and public TCRs underpin the T\u2010cell response to all the immunodominant gluten epitopes in CeD suggests that such T cells are promising diagnostic and therapeutic targets.<\/jats:p>","DOI":"10.1002\/eji.201948253","type":"journal-article","created":{"date-parts":[[2019,10,19]],"date-time":"2019-10-19T10:08:11Z","timestamp":1571479691000},"page":"256-269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["On the immune response to barley in celiac disease: Biased and public T\u2010cell receptor usage to a barley unique and immunodominant gluten epitope"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0165-5098","authenticated-orcid":false,"given":"Shiva","family":"Dahal\u2010Koirala","sequence":"first","affiliation":[{"name":"K. G. Jebsen Centre for Coeliac Disease Research University of Oslo  Oslo Norway"},{"name":"Department of Immunology University of Oslo and Oslo University Hospital\u2010Rikshospitalet  Oslo Norway"},{"name":"Institute of Clinical Medicine University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf S.","family":"Neumann","sequence":"additional","affiliation":[{"name":"K. G. Jebsen Centre for Coeliac Disease Research University of Oslo  Oslo Norway"},{"name":"Department of Immunology University of Oslo and Oslo University Hospital\u2010Rikshospitalet  Oslo Norway"},{"name":"Institute of Clinical Medicine University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Jahnsen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Oslo  Oslo Norway"},{"name":"Department of Gastroenterology Akershus University Hospital  L\u00f8renskog Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Knut E.A.","family":"Lundin","sequence":"additional","affiliation":[{"name":"K. G. Jebsen Centre for Coeliac Disease Research University of Oslo  Oslo Norway"},{"name":"Institute of Clinical Medicine University of Oslo  Oslo Norway"},{"name":"Department of Gastroenterology Oslo University Hospital\u2010Rikshospitalet  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8860-704X","authenticated-orcid":false,"given":"Ludvig M.","family":"Sollid","sequence":"additional","affiliation":[{"name":"K. G. 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