{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:33:26Z","timestamp":1790728406967,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Upon viral infection, stressed or damaged cells can release alarmins like IL\u201033 that act as endogenous danger signals alerting innate and adaptive immune cells. IL\u201033 coming from nonhematopoietic cells has been identified as important factor triggering the expansion of antiviral CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. In LN the critical cellular source of IL\u201033 is unknown, as is its potential cell\u2010intrinsic function as a chromatin\u2010associated factor. Using IL\u201033\u2010GFP reporter mice, we identify fibroblastic reticular cells (FRC) and lymphatic endothelial cells (LEC) as the main IL\u201033 source. In homeostasis, IL\u201033 is dispensable as a transcriptional regulator in FRC, indicating it functions mainly as released cytokine. Early during infection with lymphocytic choriomeningitis virus (LCMV) clone 13, both FRC and LEC lose IL\u201033 protein expression suggesting cytokine release, correlating timewise with IL\u201033 receptor expression by reactive CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and their greatly augmented expansion in WT versus\n                    <jats:italic>\n                      ll33\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Using mice lacking IL\u201033 selectively in FRC versus LEC, we identify FRC as key IL\u201033 source driving acute and chronic antiviral T\u2010cell responses. Collectively, these findings show that LN T\u2010zone FRC not only regulate the homeostasis of na\u00efve T cells but also their expansion and differentiation several days into an antiviral response.\n                  <\/jats:p>","DOI":"10.1002\/eji.201948413","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T01:55:21Z","timestamp":1595469321000},"page":"76-90","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Fibroblast\u2010derived IL\u201033 is dispensable for lymph node homeostasis but critical for CD8 T\u2010cell responses to acute and chronic viral infection"],"prefix":"10.1002","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6089-4136","authenticated-orcid":false,"given":"Patricia","family":"Aparicio\u2010Domingo","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Cannelle","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew B.","family":"Buechler","sequence":"additional","affiliation":[{"name":"Department of Cancer Immunology Genentech  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M.","family":"Kallert","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Division of Experimental Virology University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Favre","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagham","family":"Alouche","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Papazian","sequence":"additional","affiliation":[{"name":"Department of Hematology Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burkhard","family":"Ludewig","sequence":"additional","affiliation":[{"name":"Institute of Immunobiology Kantonsspital St.Gallen  St. Gallen Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1095-4273","authenticated-orcid":false,"given":"Tom","family":"Cupedo","sequence":"additional","affiliation":[{"name":"Department of Hematology Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel D.","family":"Pinschewer","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Division of Experimental Virology University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon J.","family":"Turley","sequence":"additional","affiliation":[{"name":"Department of Cancer Immunology Genentech  South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8758-957X","authenticated-orcid":false,"given":"Sanjiv A.","family":"Luther","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.06.006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.95"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12619"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215418"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1418549112"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003331"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101977"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2262"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606854104"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.04.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161126063"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.10.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.145"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34255"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05485-x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900408"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.11.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.006"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12748"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2000.00618.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712628115"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.23.12694"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1513"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15327"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003059107"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63631-0"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.8.4911-4927.2003"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.09.006"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312585111"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101724"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.038"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1605"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1144830"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702579104"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.03.012"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000072"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2014.02.012"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02238"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.10.011"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14738"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.298703"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.02.011"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424236112"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58050"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7563.1"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-2-r29"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(91)90018-U"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201948413","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201948413","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eji.201948413","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201948413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T12:39:49Z","timestamp":1693658389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201948413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,5]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/eji.201948413"],"URL":"https:\/\/doi.org\/10.1002\/eji.201948413","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/EJI.201948413\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.201948413\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.201948413\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.201948413\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.201948413\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/EJI.201948413\/v2\/review1","asserted-by":"object"}]},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,5]]},"assertion":[{"value":"2019-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}