{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:51:38Z","timestamp":1776693098803,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004442","name":"Narodowym Centrum Nauki","doi-asserted-by":"publisher","award":["2015\/16\/S\/NZ6\/00041"],"award-info":[{"award-number":["2015\/16\/S\/NZ6\/00041"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dysregulation in type I IFN and IFN\u2010stimulated genes (ISGs) induced by monocytes is one of the key features of systemic sclerosis (SSc) pathogenesis. Abnormalities in microRNA (miRNA) expression are related to excessive IFN production, however the role of miRNA remains largely elusive in SSc monocytes. This study explores global miRNA\u2010mRNA profiling of SSc monocytes and functional attenuation of IFN and ISGs by specific miRNAs. Global sequencing of mRNA (mRNA\u2010seq) and miRNA (miRNA\u2010seq) samples were performed simultaneously on healthy controls and SSc monocytes. Following computational analysis, selected miRNAs\u2013mRNA candidates were validated, correlated with clinical parameters, and tested by functional assays. Transcriptomics data and qPCR analysis confirmed IFN signature in SSc but not in rheumatoid arthritis monocytes. Based on miRNA\u2010seq analysis, five miRNAs were selected for further validation. Only the expression patterns of miRNA\u201026a\u20102\u20103p and miRNA\u2010485\u20103p were confirmed and negatively correlated with clinical parameters. Exogenous delivery of miRNA\u201026a\u20102\u20103p to TLR\u2010stimulated monocytic THP\u20101 cells specifically inhibited ISGs but not inflammasome activity in functional assays. In conclusion, our miRNA\u2013mRNA co\u2010sequencing and functional analysis identify miRNA\u201026a\u20102\u20103p as a new candidate, which is predicated to negatively regulate ISGs. This implies that reduced expression of miRNA\u201026a\u20102\u20103 may be involved in pathogenic IFN signature in SSc monocytes.<\/jats:p>","DOI":"10.1002\/eji.201948428","type":"journal-article","created":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T14:55:35Z","timestamp":1582383335000},"page":"1057-1066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Global miRNA and mRNA expression profiles identify miRNA\u201026a\u20102\u20103p\u2010dependent repression of IFN signature in systemic sclerosis human monocytes"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0961-6130","authenticated-orcid":false,"given":"Marzena","family":"Ciechomska","sequence":"first","affiliation":[{"name":"National Institute of Geriatrics Rheumatology and Rehabilitation  Warsaw 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