{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:19:18Z","timestamp":1776464358978,"version":"3.51.2"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG STE 1022\/2\u20103"],"award-info":[{"award-number":["DFG STE 1022\/2\u20103"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG STE 1022\/4\u20101"],"award-info":[{"award-number":["DFG STE 1022\/4\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201606230249"],"award-info":[{"award-number":["201606230249"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Thromboxane (TX) A\n                    <jats:sub>2<\/jats:sub>\n                    has been identified as an important intrahepatic vasoconstrictor upon Kupffer cell (KC) activation during infections such as spontaneous bacterial peritonitis (SBP). The study aimed to investigate the role of TLRs in the TXA\n                    <jats:sub>2<\/jats:sub>\n                    increase in liver nonparenchymal cells and their related mechanisms. Here, we identified TLR\u20102 as a common pathway for different microbials: microbial lysates including Gram\u2010positive bacteria, Gram\u2010negative bacteria, and fungi all increased TXA\n                    <jats:sub>2<\/jats:sub>\n                    secretion via activation of TLR\u20102 in human KCs, accompanied by increased expression and phosphorylation of Myd88\u2010related pathway. Of all TLR agonists, only TLR\u20101, \u20102, and \u20104 agonists increased TXA\n                    <jats:sub>2<\/jats:sub>\n                    in human KCs. These results were further confirmed by mouse liver nonparenchymal cells. Comparing the effects of TLR\u20101, \u20102, and \u20104 antagonists, only TLR\u20102 antagonist showed inhibitory effects with all tested microbial lysates. Pretreatment with TLR\u20102 antagonist in human KCs blocked the secretion of IL\u201010, CXCL\u201010, TNF\u2010\u03b1, and IL\u20106 induced by Gram\u2010positive and Gram\u2010negative bacterial stimulation. IL\u201023 and IL\u20101\u03b2 were only induced by Gram\u2010negative bacteria. Thus, TLR\u20102 might be a potential marker and an attractive target for future treatment of patients with SBP. In addition, IL\u201023 and IL\u20101\u03b2 might distinguish early between Gram\u2010positive and Gram\u2010negative SBP.\n                  <\/jats:p>","DOI":"10.1002\/eji.201948507","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T05:18:30Z","timestamp":1593753510000},"page":"1988-1997","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Kupffer cell activation by different microbial lysates: Toll\u2010like receptor\u20102 plays pivotal role on thromboxane A\n                    <sub>2<\/sub>\n                    production in mice and humans"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3346-2810","authenticated-orcid":false,"given":"Jiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Medicine II University Hospital Liver Centre Munich LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Wieser","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Medical Microbiology and Hospital Epidemiology Max von Pettenkofer Institute LMU Munich  Munich Germany"},{"name":"Division of Infectious Diseases and Tropical Medicine University Hospital LMU Munich  Munich Germany"},{"name":"German Center for Infection Research (DZIF) Partner Site Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medicine II University Hospital Liver Centre Munich LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanwei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medicine II University Hospital Liver Centre Munich LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moyan","family":"Hu","sequence":"additional","affiliation":[{"name":"Chair for Fish Diseases and Fisheries Biology Faculty of Veterinary Medicine Ludwig\u2010Maximilians\u2010University of Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ina\u2010Kristin","family":"Behrens","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Medical Microbiology and Hospital Epidemiology Max von Pettenkofer Institute LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias S.","family":"Schiergens","sequence":"additional","affiliation":[{"name":"Department of General, Visceral, and Transplant Surgery Ludwig\u2010Maximilians\u2010University Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander L.","family":"Gerbes","sequence":"additional","affiliation":[{"name":"Department of Medicine II University Hospital Liver Centre Munich LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian J.","family":"Steib","sequence":"additional","affiliation":[{"name":"Department of Medicine II University Hospital Liver Centre Munich LMU Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-314184"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000000927"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000375352"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23596"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489966"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr.15027419"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1572-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2015.91"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2019.12.013"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH09-07-0442"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20860"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.11.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2012.01838.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b01691"},{"key":"e_1_2_8_16_1","first-page":"CR144","article-title":"Prognostic significance of platelet function in the early phase of ST\u2010elevation myocardial infarction treated with primary angioplasty","volume":"14","author":"Huczek Z.","year":"2008","journal-title":"Med. 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