{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T03:54:21Z","timestamp":1781754861098,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Immunopathogenic roles for both Th1 (CD4\n                    <jats:sup>+<\/jats:sup>\n                    IFN\u2010\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    ) and Th17 (CD4\n                    <jats:sup>+<\/jats:sup>\n                    IL\u201017A\n                    <jats:sup>+<\/jats:sup>\n                    ) cells have been demonstrated in experimental autoimmune uveitis (EAU). However, the role for Th17\/Th1 (CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells co\u2010expressing IFN\u2010\u03b3 and IL\u201017A) cells in EAU is not yet understood. Using interphotoreceptor retinoid\u2010binding protein peptide\u2010induced EAU in mice, we found increased levels of Th17\/Th1 cells in EAU retinae (mean 9.6\u00a0\u00b1\u00a04.2%) and draining LNs (mean 8.4\u00a0\u00b1\u00a03.9%;\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.01) relative to controls. Topical dexamethasone treatment effectively reduced EAU severity and decreased retinal Th1 cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.01), but had no impact on retinal Th17\/Th1 or Th17 cells compared to saline controls. Using in vitro migration assays with mouse CNS endothelium, we demonstrated that Th17\/Th1 cells were significantly increased within the migrated population relative to controls (mean 15.6\u00a0\u00b1\u00a09.5% vs. 1.9\u00a0\u00b1\u00a01.5%;\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.01). Chemokine receptor profiles of Th17\/Th1 cells (CXCR3 and CCR6) did not change throughout the transendothelial migration process and were unaffected by dexamethasone treatment. These findings support a role for Th17\/Th1 cells in EAU and their resistance to steroid inhibition suggests the importance of targeting both Th17 and Th17\/Th1 cells for improving therapy.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048616","type":"journal-article","created":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T16:43:31Z","timestamp":1594485811000},"page":"1941-1951","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Functionally distinct IFN\u2010\u03b3\n                    <sup>+<\/sup>\n                    IL\u201017A\n                    <sup>+<\/sup>\n                    Th cells in experimental autoimmune uveitis: T\u2010cell heterogeneity, migration, and steroid response"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2682-939X","authenticated-orcid":false,"given":"Yi\u2010Hsing","family":"Chen","sequence":"first","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"},{"name":"Department of Ophthalmology Chang Gung Memorial Hospital  Taoyuan Taiwan"},{"name":"College of Medicine Chang Gung University  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malihe","family":"Eskandarpour","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelia","family":"Gondrand","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renyang","family":"Gu","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grazyna","family":"Galatowicz","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue L.","family":"Lightman","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"},{"name":"Moorfields Eye Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1422-2926","authenticated-orcid":false,"given":"Virginia L.","family":"Calder","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-014-0433-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/09273949409057071"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071258"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/521957"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.18"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242648"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029421"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13124"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201847677"},{"key":"e_1_2_7_12_1","first-page":"1","article-title":"Increased plasticity of non\u2010classic Th1 cells towards the Th17 phenotype","author":"Leipe J.","year":"2019","journal-title":"Mod. 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