{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T07:27:02Z","timestamp":1787642822072,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T00:00:00Z","timestamp":1593475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recent studies have implicated a role for adenosine\u2010dependent immunosuppression in head and neck tumor microenvironments. We describe expression of CD73, an enzyme critical to the generation of adenosine from extracellular AMP, in T cells and other cell types within human head and neck tumors. Flow cytometric analyses of tumor\u2010infiltrating cells indicate that CD3\n                    <jats:sup>+<\/jats:sup>\n                    cells are the predominant source of CD73 among immune infiltrating cells and that CD73 expression, especially among CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, is inversely related to indices of T cell infiltration and T cell activation in the microenvironment of head and neck tumors. We provide evidence that CD73 expression on peripheral T cells and levels of soluble CD73 in circulation are correlated with CD73 expression on CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in tumors. Moreover, fluorescent microscopy studies reveal that CD8\n                    <jats:sup>+<\/jats:sup>\n                    CD73\n                    <jats:sup>+<\/jats:sup>\n                    cells are observed in close proximity to tumor cells as well as in surrounding tissue. In vitro studies with peripheral blood T cells indicate that anti\u2010CD3\u2010stimulation causes loss of CD73 expression, especially among cells that undergo proliferation and that exogenous AMP can impair T cell proliferation, while sustaining CD73 expression. These data suggest that CD8\n                    <jats:sup>+<\/jats:sup>\n                    CD73\n                    <jats:sup>+<\/jats:sup>\n                    T cells may be especially important mediators of immunosuppression in human head and neck cancer.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048626","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T02:35:45Z","timestamp":1592361345000},"page":"2055-2066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["CD8\n                    <sup>+<\/sup>\n                    CD73\n                    <sup>+<\/sup>\n                    T cells in the tumor microenvironment of head and neck cancer patients are linked to diminished T cell infiltration and activation in tumor tissue"],"prefix":"10.1002","volume":"50","author":[{"given":"Soumya","family":"Panigrahi","sequence":"first","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas A.","family":"Bazdar","sequence":"additional","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwah","family":"Albakri","sequence":"additional","affiliation":[{"name":"Department of Pathology Case Western Reserve University School of Medicine  Cleveland Ohio"},{"name":"Taibah University KSA, College of Applied Medical Sciences Department of Medical Laboratory Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Freeman","sequence":"additional","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Dubyak","sequence":"additional","affiliation":[{"name":"Case Western Reserve University School of Medicine Department of Physiology and Biophysics  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad","family":"Zender","sequence":"additional","affiliation":[{"name":"MED\u2010Otolaryngology University of Cincinnati  Cincinnati Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1769-8682","authenticated-orcid":false,"given":"Scott F.","family":"Sieg","sequence":"additional","affiliation":[{"name":"Case Western Reserve School of Medicine Division of Infectious Diseases and HIV Medicine  Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2009-0289"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.68.1478"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.1509"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1602252"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-15-0210"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13954"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-001-0042-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-005-5050-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2012.04623.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2016.04.001"},{"key":"e_1_2_7_13_1","article-title":"Targeting the CD73\u2010adenosine axis in immuno\u2010oncology","author":"Allard D.","year":"2018","journal-title":"Immunol. 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