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The latter can cause severe nosocomial infections, with outbreaks of multi\u2010antibiotics resistant strains, thus being a major public health threat. In this study, we report that Enterobacteriaceae\u2010reactive memory Th cells were highly enriched in a CCR6\n                    <jats:sup>+<\/jats:sup>\n                    CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    Th1*\/17 cell subset and produced IFN\u2010\u03b3, IL\u201017A, and IL\u201022. This T cell subset was severely reduced in septic patients with\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    bloodstream infection who also selectively lacked circulating\n                    <jats:italic>K. pneumonie<\/jats:italic>\n                    \u2010reactive T cells. By combining heterologous antigenic stimulation, single cell cloning and TCR V\u03b2 sequencing, we demonstrate that a large fraction of memory Th cell clones was broadly cross\u2010reactive to several Enterobacteriaceae species. These cross\u2010reactive Th cell clones were expanded in vivo and a large fraction of them recognized the conserved outer membrane protein A antigen. Interestingly, Enterobacteriaceae broadly cross\u2010reactive T cells were also prominent among in vitro primed na\u00efve T cells. Collectively, these data point to the existence of immunodominant T cell epitopes shared among different Enterobacteriaceae species and targeted by cross\u2010reactive T cells that are readily found in the pre\u2010immune repertoire and are clonally expanded in the memory repertoire.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048630","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T22:57:54Z","timestamp":1606172274000},"page":"648-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Broadly reactive human CD4\n                    <sup>+<\/sup>\n                    T cells against Enterobacteriaceae are found in the na\u00efve repertoire and are clonally expanded in the memory 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