{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T08:35:27Z","timestamp":1782462927421,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The amplitude and duration of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling is crucial for B\u2010cell development and self\u2010tolerance; however, the mechanisms for terminating Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals in B cells have not been determined. In lymphocytes, plasma membrane Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ATPase (PMCA) isoforms 1 and 4 (PMCA1 and PMCA4, aka ATP2B1 and ATP2B4) are the main candidates for expelling Ca\n                    <jats:sup>2+<\/jats:sup>\n                    from the cell through the plasma membrane. We report here that\n                    <jats:italic>Pmca4<\/jats:italic>\n                    (\n                    <jats:italic>Atp2b4<\/jats:italic>\n                    ) KO mice had normal B\u2010cell development, while mice with a conditional KO of\n                    <jats:italic>Pmca1<\/jats:italic>\n                    (\n                    <jats:italic>Atp2b1<\/jats:italic>\n                    ) had greatly reduced numbers of B cells, particularly splenic follicular B cells, marginal zone B cells, and peritoneal B\u20101a cells. Mouse and na\u00efve human B cells showed only PMCA1 expression and no PMCA4 by western blot, in contrast to T cells, which did express PMCA4. Calcium handling was normal in\n                    <jats:italic>\n                      Pmca4\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    B cells, but\n                    <jats:italic>Pmca1<\/jats:italic>\n                    KO B cells had elevated basal levels of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , elevated levels in ER stores, and reduced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    clearance. These findings show that the PMCA1 isoform alone is required to ensure normal B\u2010cell Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling and development, which may have implications for therapeutic targeting of PMCAs and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in B cells.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048654","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T13:57:56Z","timestamp":1603547876000},"page":"594-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Plasma membrane Ca\n                    <sup>2+<\/sup>\n                    ATPase 1 (PMCA1) but not PMCA4 is critical for B\u2010cell development and Ca\n                    <sup>2+<\/sup>\n                    homeostasis in mice"],"prefix":"10.1002","volume":"51","author":[{"given":"Mark","family":"Korthals","sequence":"first","affiliation":[{"name":"Institute for Biochemistry and Cell Biology Otto\u2010von\u2010Guericke University  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Tech","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Cell Biology Otto\u2010von\u2010Guericke University  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Langnaese","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Cell Biology Otto\u2010von\u2010Guericke University  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gottfried","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Cell Biology Otto\u2010von\u2010Guericke University  Magdeburg 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