{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:09:06Z","timestamp":1791889746174,"version":"4.3.4"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.186.116"],"award-info":[{"award-number":["016.186.116"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["917.13.338"],"award-info":[{"award-number":["917.13.338"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009425","name":"Landsteiner Foundation for Blood Transfusion Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009425","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tissue\u2010resident memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (T\n                    <jats:sub>RM<\/jats:sub>\n                    ) localize to barrier tissues and mediate local protection against reinvading pathogens. Circulating central memory (T\n                    <jats:sub>CM<\/jats:sub>\n                    ) and effector memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (T\n                    <jats:sub>EM<\/jats:sub>\n                    ) also contribute to tissue recall responses, but their potential to form mucosal T\n                    <jats:sub>RM<\/jats:sub>\n                    remains unclear. Here, we employed adoptive transfer and lymphocytic choriomeningitis virus reinfection models to specifically assess secondary responses of T\n                    <jats:sub>CM<\/jats:sub>\n                    and T\n                    <jats:sub>EM<\/jats:sub>\n                    at mucosal sites. Donor T\n                    <jats:sub>CM<\/jats:sub>\n                    and T\n                    <jats:sub>EM<\/jats:sub>\n                    exhibited robust systemic recall responses, but only limited accumulation in the small intestine, consistent with reduced expression of tissue\u2010homing and \u2010retention molecules. Murine and human circulating memory T cells also exhibited limited CD103 upregulation following TGF\u2010\u03b2 stimulation. Upon pathogen clearance, T\n                    <jats:sub>CM<\/jats:sub>\n                    and T\n                    <jats:sub>EM<\/jats:sub>\n                    readily gave rise to secondary T\n                    <jats:sub>EM<\/jats:sub>\n                    . T\n                    <jats:sub>CM<\/jats:sub>\n                    also formed secondary central memory in lymphoid tissues and T\n                    <jats:sub>RM<\/jats:sub>\n                    in internal tissues, for example, the liver. Both T\n                    <jats:sub>CM<\/jats:sub>\n                    and T\n                    <jats:sub>EM<\/jats:sub>\n                    failed to substantially contribute to resident mucosal memory in the small intestine, while activated intestinal T\n                    <jats:sub>RM<\/jats:sub>\n                    , but not liver T\n                    <jats:sub>RM<\/jats:sub>\n                    , efficiently reformed CD103\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>RM<\/jats:sub>\n                    . Our findings demonstrate that circulating T\n                    <jats:sub>CM<\/jats:sub>\n                    and T\n                    <jats:sub>EM<\/jats:sub>\n                    are limited in generating mucosal T\n                    <jats:sub>RM<\/jats:sub>\n                    upon reinfection. This may pose important implications on cell therapy and vaccination strategies employing memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells for protection at mucosal sites.\n                  <\/jats:p>","DOI":"10.1002\/eji.202048737","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T21:47:55Z","timestamp":1596750475000},"page":"151-166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Circulating memory CD8\n                    <sup>+<\/sup>\n                    T cells are limited in forming CD103\n                    <sup>+<\/sup>\n                    tissue\u2010resident memory T cells at mucosal sites after reinfection"],"prefix":"10.1002","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1158-5011","authenticated-orcid":false,"given":"Felix M.","family":"Behr","sequence":"first","affiliation":[{"name":"Department of Hematopoiesis Sanquin Research and Landsteiner Laboratory Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Department of Experimental Immunology Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammarina","family":"Beumer\u2010Chuwonpad","sequence":"additional","affiliation":[{"name":"Department of Hematopoiesis Sanquin Research and Landsteiner Laboratory Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasja A. M.","family":"Kragten","sequence":"additional","affiliation":[{"name":"Department of Hematopoiesis Sanquin Research and Landsteiner Laboratory Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Wesselink","sequence":"additional","affiliation":[{"name":"Department of Hematopoiesis Sanquin Research and Landsteiner Laboratory Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7693-799X","authenticated-orcid":false,"given":"Regina","family":"Stark","sequence":"additional","affiliation":[{"name":"Department of Hematopoiesis Sanquin Research and Landsteiner Laboratory Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"Department of Experimental Immunology Amsterdam UMC University of Amsterdam  Amsterdam The Netherlands"},{"name":"BIH Center for Regenerative Therapies Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3623-7555","authenticated-orcid":false,"given":"Klaas P. J. 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