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There is much interest in using Tregs as a cell therapy to re\u2010establish tolerance in conditions such as inflammatory bowel disease and type 1 diabetes, with many ongoing clinical studies testing the safety and efficacy of this approach. Manufacturing of Tregs for therapy typically involves <jats:italic>ex vivo<\/jats:italic> expansion to obtain sufficient cell numbers for infusion and comes with the risk of altering the activity of key biological processes. However, this process also offers an opportunity to tailor Treg function to maximize <jats:italic>in vivo<\/jats:italic> activity. In this review, we focus on the roles of antigen\u2010presenting cells (APCs) in the generation and function of Tregs in humans. In addition to stimulating the development of Tregs, APCs activate Tregs and provide signals that induce specialized functional and homing marker expression. Cross talk between Tregs and APCs is a critical, often under\u2010appreciated, aspect of Treg biology, with APCs mediating the key properties of infectious tolerance and bystander suppression. Understanding the biology of human Treg\u2010APC interactions will reveal new ways to optimize Treg\u2010based therapeutic approaches.<\/jats:p>","DOI":"10.1002\/eji.202048746","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T01:38:40Z","timestamp":1607650720000},"page":"27-38","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Cross talk between human regulatory T cells and antigen\u2010presenting cells: Lessons for clinical applications"],"prefix":"10.1002","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2418-5076","authenticated-orcid":false,"given":"Christine M","family":"Wardell","sequence":"first","affiliation":[{"name":"Department of Surgery University of British Columbia  Vancouver British Columbia Canada"},{"name":"BC Children's Hospital Research Institute University of British Columbia  Vancouver British Columbia 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